Luteolin Possess Anti-inflammatory Effect on LPS Induced RAW 264,7 Cell Lines

Ervi Afifah, Hartini Tiono, Philips Onggowidjaja, Selonan Obeng, Wahyu Widowati, Cintani Wahyuni, Cahyaning Wijayanti, Muhammad Maulana, Tri Handayani, Rizal Rizal, Rizal Rizal

2021

Abstract

BACKGROUND: Inflammation is a natural human reaction to potentially harmful effects such as tissue stress, trauma, and microbial infection. Extended inflammation is believed related to several chronic conditions, involving asthma, rheumatoid arthritis and even cancer. To avoid immune cells from causing more tissue damage, inflammatory responses must be regulated. Anti-inflammatory agents are particularly beneficial for these purposes. Luteolin is flavonoid and has potent anti-inflammatory effects. OBJECTIVE: The study aimed to determine anti-inflammatory effect of luteolin on LPS induced RAW 264,7 cell lines. METHOD: The MTS assay was used to determine the viability of cells and the nontoxic concentration of cell lines. The anti-inflammatory activity was assessed with Elisa assay of inflammatory parameters including PGE-2, TNF-α, and IL-1β using secreted cytokine levels in culture supernatants of RAW 264,7 cell line. RESULT: The toxic concentration of luteolin was 100 μM/mL, so that the concentration was not used for treatment. Concentrations of 4 and 20 μM/mL demonstrated high viability (>90\%), they were suitable for treatment. Luteolin 4 µM/mL significantly increased the inhibition of inflammatory cytokines PGE-2, TNF-α and IL-1β compared to positive control. CONCLUSION: The research reported that Luteolin possesses the anti-inflammatory effect indicated by properties of inflammatory inhibition toward PGE-2, TNF-α and IL-1β.

Paper Citation

in Harvard Style

Afifah E., Tiono H., Onggowidjaja P., Obeng S., Widowati W., Wahyuni C., Wijayanti C., Maulana M., Handayani T. and Rizal R. (2021). Luteolin Possess Anti-inflammatory Effect on LPS Induced RAW 264,7 Cell Lines. In Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - Volume 1: ICE-TES, ISBN 978-989-758-601-9, pages 74-80. DOI: 10.5220/0010744500003113

in Bibtex Style

@conference{ice-tes21,
author={Ervi Afifah and Hartini Tiono and Philips Onggowidjaja and Selonan Obeng and Wahyu Widowati and Cintani Wahyuni and Cahyaning Wijayanti and Muhammad Maulana and Tri Handayani and Rizal Rizal},
title={Luteolin Possess Anti-inflammatory Effect on LPS Induced RAW 264,7 Cell Lines},
booktitle={Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - Volume 1: ICE-TES,},
year={2021},
pages={74-80},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010744500003113},
isbn={978-989-758-601-9},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - Volume 1: ICE-TES,
TI - Luteolin Possess Anti-inflammatory Effect on LPS Induced RAW 264,7 Cell Lines
SN - 978-989-758-601-9
AU - Afifah E.
AU - Tiono H.
AU - Onggowidjaja P.
AU - Obeng S.
AU - Widowati W.
AU - Wahyuni C.
AU - Wijayanti C.
AU - Maulana M.
AU - Handayani T.
AU - Rizal R.
PY - 2021
SP - 74
EP - 80
DO - 10.5220/0010744500003113