The Effect of Agarwood Leaves Ethanol Extract on Porphyromonas gingivalis Growth Inhibition and in Vitro Cytotoxicity Assay on Fibroblast

Vinna Sugiaman, Henry Mandalas, Ethan Tanamal, Nathalia Calista, Natallia Pranata

2021

Abstract

Agarwood leaves (Aquilaria malaccensis Lamk.) have an antibacterial activity that could be used as wound healing agent. Porphyromonas gingivalis were the main pathogens in periodontitis. This was a laboratory experimental study post-test only control group design. Agarwood leaves were obtained from Ibun Garden, Majalaya District, West Java. Sample identified and determined by Biology Research Center, LIPI Indonesia. Extraction and phytochemical test were conducted at Aretha Medika Utama BBRC Bandung and BPTRO Bogor. Fibroblast ATCC 3T3 Balb/C obtained and cultured at Aretha Medika Utama BBRC. Cytotoxicity test was carried out by using MTS Assay method, and the results are adjusted to ISO 10993-5. IC50 was obtained using PROBIT analysis. Inhibition assay was carried out by well-diffusion method and the results are adjusted to Davis and Stout criteria. Research and P. gingivalis (ATCC 33277) was carried out at Microbiology Laboratory Faculty of Dentistry, Universitas Padjadjaran. Results were analysed with ANOVA. The results indicate agarwood leaves had weak inhibitory ability at under concentration of 50% and moderate inhibition at a concentration of 100%. The cytotoxicity results showed no toxic effect at under concentrations of 62.5 µg/mL. The IC50 at a concentration of 215.54 µg/mL.

Paper Citation

in Harvard Style

Sugiaman V., Mandalas H., Tanamal E., Calista N. and Pranata N. (2021). The Effect of Agarwood Leaves Ethanol Extract on Porphyromonas gingivalis Growth Inhibition and in Vitro Cytotoxicity Assay on Fibroblast. In Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - Volume 1: ICE-TES, ISBN 978-989-758-601-9, pages 112-121. DOI: 10.5220/0010745500003113

in Bibtex Style

@conference{ice-tes21,
author={Vinna Sugiaman and Henry Mandalas and Ethan Tanamal and Nathalia Calista and Natallia Pranata},
title={The Effect of Agarwood Leaves Ethanol Extract on Porphyromonas gingivalis Growth Inhibition and in Vitro Cytotoxicity Assay on Fibroblast},
booktitle={Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - Volume 1: ICE-TES,},
year={2021},
pages={112-121},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010745500003113},
isbn={978-989-758-601-9},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - Volume 1: ICE-TES,
TI - The Effect of Agarwood Leaves Ethanol Extract on Porphyromonas gingivalis Growth Inhibition and in Vitro Cytotoxicity Assay on Fibroblast
SN - 978-989-758-601-9
AU - Sugiaman V.
AU - Mandalas H.
AU - Tanamal E.
AU - Calista N.
AU - Pranata N.
PY - 2021
SP - 112
EP - 121
DO - 10.5220/0010745500003113