Application of Freeze-thaw Harvest for SARS-CoV-2 PCR EQA Panel Material

Nur Hariastuti, Nike Susanti, Hana Pawestri, Kartika Puspa

2021

Abstract

BACKGROUND: Currently there are more than 700 testing laboratories for COVID-19 in Indonesia. To ensure that the laboratory has a good performance, a proficiency test panel for external quality assurance program was conducted. The production of panels derived from virus isolate from cell culture, generally does not contain ribonuclease protein (RNP) as in clinical samples. OBJECTIVE: To generate a panel that resembles clinical samples, we conducted experiments to produce panels containing RNP by freeze-thaw protocol. METHODS: SARS-CoV-2 virus cultures were performed in the NIHRD BSL-3 laboratory facility. Harvesting is carried out on the 3rd, 6th, and 9th days with: no freeze-thaw process, 1 freeze-thaw process, 2 freeze-thaw processes, and 3 freeze-thaw processes. RESULTS: On the 3rd day of observation, the Ct isolates had reached an average of 11.53 and did not increase with the increase in incubation time. Meanwhile, the viral Ct became smaller in the presence of freeze-thaw treatment. RNP began to be detected on day 3 with an average Ct of 35.40 and improving with the addition of days and the number of freeze-thaw treatments. CONCLUSION: Freeze-thaw treatment can be used to improve the value of Ct however, the detected Ct RNP value was still higher than the viral Ct.

Paper Citation

in Harvard Style

Hariastuti N., Susanti N., Pawestri H. and Puspa K. (2021). Application of Freeze-thaw Harvest for SARS-CoV-2 PCR EQA Panel Material. In Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - Volume 1: ICE-TES, ISBN 978-989-758-601-9, pages 250-253. DOI: 10.5220/0010748800003113

in Bibtex Style

@conference{ice-tes21,
author={Nur Hariastuti and Nike Susanti and Hana Pawestri and Kartika Puspa},
title={Application of Freeze-thaw Harvest for SARS-CoV-2 PCR EQA Panel Material},
booktitle={Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - Volume 1: ICE-TES,},
year={2021},
pages={250-253},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010748800003113},
isbn={978-989-758-601-9},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - Volume 1: ICE-TES,
TI - Application of Freeze-thaw Harvest for SARS-CoV-2 PCR EQA Panel Material
SN - 978-989-758-601-9
AU - Hariastuti N.
AU - Susanti N.
AU - Pawestri H.
AU - Puspa K.
PY - 2021
SP - 250
EP - 253
DO - 10.5220/0010748800003113