Study on HPLC Fingerprint and Chemical Pattern Recognition of Guizhi Shaoyao Zhimu Granules

Shujing Zheng, Zhenglong Li, Jia Wang, Yang Li, Xiangyang Leng

2022

Abstract

Objective: To establish the fingerprint of Guizhi Shaoyao Zhimu Granules, and evaluate its quality in combination with the chemical pattern recognition method, so as to provide a reference for the quality control of the preparation. Method: Use Waters XSelect HSS T3 (4.6 mm ×250 mm, 5 µm) chromatographic column; mobile phase is acetonitrile-0.1% phosphoric acid aqueous solution; gradient elution; flow rate is 1.0 min·m L–1; column temperature is 35ºC; the detection wavelength was 210 nm; the injection volume was 10 µL. Ten batches of Guizhi Shaoyao Zhimu Granules fingerprints were established, and similarity evaluation, cluster analysis, principal component analysis and orthogonal partial least squares discriminant analysis were performed. Results: The established HPLC fingerprint of Guizhi Shaoyao Zhimu Granules identified 16 common peaks, and identified ephedrine hydrochloride, D-pseudu-ephedrine hydrochloride, mangiferin, paeoniflorin, liquiritin, and 5-O-methylvisammioside , cinnamic acid, glycyrrhizic acid, 6-ginger phenol; 9 batches of Guizhi Shaoyao Zhimu Granules fingerprint pattern and control pattern similarity are all> 0.990, cluster analysis and principal component analysis results are basically the same, the orthogonality is the smallest. The two-fold discriminant analysis method screened out 9 quality difference markers. Conclusion: The method is simple and reliable, and can provide a reference for the quality control of Guizhi Shaoyao Zhimu Granules.

Paper Citation

in Harvard Style

Zheng S., Li Z., Wang J., Li Y. and Leng X. (2022). Study on HPLC Fingerprint and Chemical Pattern Recognition of Guizhi Shaoyao Zhimu Granules. In Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB, ISBN 978-989-758-595-1, pages 139-145. DOI: 10.5220/0011191700003443

in Bibtex Style

@conference{icbeb22,
author={Shujing Zheng and Zhenglong Li and Jia Wang and Yang Li and Xiangyang Leng},
title={Study on HPLC Fingerprint and Chemical Pattern Recognition of Guizhi Shaoyao Zhimu Granules},
booktitle={Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,},
year={2022},
pages={139-145},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0011191700003443},
isbn={978-989-758-595-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,
TI - Study on HPLC Fingerprint and Chemical Pattern Recognition of Guizhi Shaoyao Zhimu Granules
SN - 978-989-758-595-1
AU - Zheng S.
AU - Li Z.
AU - Wang J.
AU - Li Y.
AU - Leng X.
PY - 2022
SP - 139
EP - 145
DO - 10.5220/0011191700003443