The Activity of Microalgae in Ballast Water based on Microfluidic Chip Electrokinetic Technology

Runzhe Sun, Zhen Li, Zhen Liu, Na Li, Yongxin Song

2022

Abstract

To control the marine bio-invasions in ballast water, the regulatory discharge standards specify the number of viable organisms in ballast water treatment. It is an important task to determine the activity of microalgae after ballast water treatment. In the current study, five kinds of microalgae were detected on microfluidic chip. The electrokinetic velocity (EV) and diameter of microalgae were measured manually using an optical microscope. Finally, the contour detection and Lucas-Kanada (L-K) Optical Flow technique were used to calculate the diameter and velocity of microalgae, respectively. The result found that the EV of different species of living microalgae decreases with increasing diameter. The EV of dead Pyramimonas sp., Platymonas and Prorocentrum donghaiense decreased to 0 µm/s. In addition, the L-K optical flow technique can obtain the movement velocity of microalgae at any time, which can improve the detection accuracy. Those study demonstrate that the development of a new field ballast water analysis instrument based on contour detection and L-K optical flow technique is of great significance.

Paper Citation

in Harvard Style

Sun R., Li Z., Liu Z., Li N. and Song Y. (2022). The Activity of Microalgae in Ballast Water based on Microfluidic Chip Electrokinetic Technology. In Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB, ISBN 978-989-758-595-1, pages 283-287. DOI: 10.5220/0011201100003443

in Bibtex Style

@conference{icbeb22,
author={Runzhe Sun and Zhen Li and Zhen Liu and Na Li and Yongxin Song},
title={The Activity of Microalgae in Ballast Water based on Microfluidic Chip Electrokinetic Technology},
booktitle={Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,},
year={2022},
pages={283-287},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0011201100003443},
isbn={978-989-758-595-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,
TI - The Activity of Microalgae in Ballast Water based on Microfluidic Chip Electrokinetic Technology
SN - 978-989-758-595-1
AU - Sun R.
AU - Li Z.
AU - Liu Z.
AU - Li N.
AU - Song Y.
PY - 2022
SP - 283
EP - 287
DO - 10.5220/0011201100003443