The Investigation of P7 Peptide Delivery Targeting Cdc24 in Ras-driven Pancreatic Cancer by L-fucose-bound Liposome

Yurui Yang, Hongbin Lan, Huawei Zhu, Yiran Zhu

2022

Abstract

Pancreatic adenocarcinoma is one of the most common leading causes of cancer deaths with an increasing incidence in the developed world. Most pancreatic cancers are induced by Ras protein abnormality. Since Ras is involved in many important cellular functions, targeting Ras alone is difficult with little progression. Currently, the only surgical resection offers the potential cure to this disease. However, among those who have the chance to receive surgery, most of them suffer from recurrence within a year. The previous study has developed novel peptides targeting Cell division control protein 42 (Cdc42), which is a type of Rho family small GTPase activated by Ras and achieved great success in inhibition of tumor cell growth. Since Cdc42 is also expressed in normal cells, a suitable drug delivery system is required for targeted therapy. This study investigates the cytotoxic and cell penetration effects of L-fucose-bound liposome-P7 targeting pancreatic cancer cells, in both in vitro and in vivo conditions. The experiments will use know human pancreatic cancer cell lines, human pancreatic epithelial cell lines, and Xenograft Murine Models. The concentration of P7 in each L-fucose-bound liposome will be assessed through half-log dilution. Cell proliferation and cytotoxicity are measured through colony formation assay, MTT assay, and Annexin V/ propidium iodide (PI) assay. The cell penetration effect will be reflected by fluorescence microscopy. There are three most possible results: (1) L-fucose-bound liposome-P7 inhibits the pancreatic cancer cell proliferation in both in vitro and in vivo cell lines without causing significant cytotoxicity to normal cells; (2) L-fucose-bound liposome-P7 only inhibits the cancer cell proliferation in vitro cell cultures without causing significant damage to normal cells; (3) L-fucose-bound liposome-P7 inhibits tumor cell proliferation in both normal pancreatic epithelial cells and cancer cells. The result of our study will provide important information for deciding whether to continue P7 peptide development in clinical trials. Future studies should focus on improving the drug delivery system and investigating P7 effects on transformed tumor cells.

Paper Citation

in Harvard Style

Yang Y., Lan H., Zhu H. and Zhu Y. (2022). The Investigation of P7 Peptide Delivery Targeting Cdc24 in Ras-driven Pancreatic Cancer by L-fucose-bound Liposome. In Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB, ISBN 978-989-758-595-1, pages 651-660. DOI: 10.5220/0011251700003443

in Bibtex Style

@conference{icbeb22,
author={Yurui Yang and Hongbin Lan and Huawei Zhu and Yiran Zhu},
title={The Investigation of P7 Peptide Delivery Targeting Cdc24 in Ras-driven Pancreatic Cancer by L-fucose-bound Liposome},
booktitle={Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,},
year={2022},
pages={651-660},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0011251700003443},
isbn={978-989-758-595-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,
TI - The Investigation of P7 Peptide Delivery Targeting Cdc24 in Ras-driven Pancreatic Cancer by L-fucose-bound Liposome
SN - 978-989-758-595-1
AU - Yang Y.
AU - Lan H.
AU - Zhu H.
AU - Zhu Y.
PY - 2022
SP - 651
EP - 660
DO - 10.5220/0011251700003443