Parasporal Cry Protein Parasporin-2 Produced by Bacillus thuringiensis Has in Vitro Toxicity on Human Cancer Cells (HepG2) under the Action of Proteinase K

Xuan Zhou

2022

Abstract

Liver cancer is one of the malignant tumors with the fastest increasing morbidity and mortality and the greatest threat to people’s health and life. This article investigates whether parasporin-2 produced by the hydrolytic non-hemolytic Bacillus thuriensis can recognize liver cancer cells and have cytotoxicity to them. In this paper, Bacillus thurinensis parasporal Cry protein—parasporin genotypes were determined in this strain using the PCR amplification. Then, parasporin-2 was separated by SDS-PAGE and purified. The same concentration of cultured HepG2 (human liver cancer cells) and L-O2 (normal human liver fibroblasts) was added to the plate and divided into two groups. Then, 3 solutions contain parasporin-2 and protease K was added to the plate. The damage to the cells was observed under a microscope and graded the degree of cell damage (CPE), and MTT then determined cytotoxicity (CT). Analysis the SDS-page and following conclusions may be drawn by comparing CPE and CT in each group. First, through horizontal comparison, the data of HepG2 and L-O2 cells in each group were compared, to determine whether parasporin-2 is toxic to liver cancer cells but not to ordinary liver fibroblasts. Besides, longitudinal comparison is the situation of CPE and CT in different groups, whether Parasporin-2 plus Protease K can produce toxicity on HepG2 of liver cancer cells. Bacillus thuringiensis can produce parasporin-2, and after the decomposition of protease K protein, parasporin-2 can produce recognition and cytotoxicity to liver cancer cells. analysis the degree of cell destruction and toxicity, after dealing with the proteinase k, cut parasporin – 2 toxicity is activated. Conclude that parasporin - 2 which hydrolyzed by protease k has a recognition on the liver cancer cells and it’s toxic to cancer cells but will not produce toxicity to normal liver cells.

Paper Citation

in Harvard Style

Zhou X. (2022). Parasporal Cry Protein Parasporin-2 Produced by Bacillus thuringiensis Has in Vitro Toxicity on Human Cancer Cells (HepG2) under the Action of Proteinase K. In Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB, ISBN 978-989-758-595-1, pages 723-728. DOI: 10.5220/0011264400003443

in Bibtex Style

@conference{icbeb22,
author={Xuan Zhou},
title={Parasporal Cry Protein Parasporin-2 Produced by Bacillus thuringiensis Has in Vitro Toxicity on Human Cancer Cells (HepG2) under the Action of Proteinase K},
booktitle={Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,},
year={2022},
pages={723-728},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0011264400003443},
isbn={978-989-758-595-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,
TI - Parasporal Cry Protein Parasporin-2 Produced by Bacillus thuringiensis Has in Vitro Toxicity on Human Cancer Cells (HepG2) under the Action of Proteinase K
SN - 978-989-758-595-1
AU - Zhou X.
PY - 2022
SP - 723
EP - 728
DO - 10.5220/0011264400003443