Screening and Identification of Bacillus amyloliquefaciens from the Gut of Aquatic Animals

Hedong Lu, Hedong Lu, Chengyuan Gu, Tao Yan, Qihan Zhang, Chengxin Geng, Can Lv

2022

Abstract

Probiotics have the dual effects of inhibiting pathogenic bacteria and promoting growth, while being green and environmentally friendly, and will not cause problems such as increased drug resistance, veterinary drug residue, and meat quality deterioration, making them to be good substitute for antibiotic. In order to realize healthy and ecological culture and provide good microbial resources for probiotics, this experiment isolated probiotics from the intestinal tracts of cultured Procambarus clarkii and crab. One strain LPB-5 was identified according to traditional colony morphological characteristics observation, physiological and biochemical tests and 16S rDNA sequence analysis. The results showed that the strain had the closest relationship with Bacillus amyloliquefaciens MPA 1034, the 16S rDNA sequence similarity was 99%, and the physiological and biochemical reaction spectrum was consistent with the 16S rDNA sequence analysis, which identified the strain as Bacillus amyloliquefaciens. Procambarus clarkii and crab are important aquaculture animals. The probiotics isolated from the intestinal tract of procambarus clarkii have their own biosafety and high efficiency, which lays a foundation for the later development of probiotics application in aquatic feed.

Paper Citation

in Harvard Style

Lu H., Gu C., Yan T., Zhang Q., Geng C. and Lv C. (2022). Screening and Identification of Bacillus amyloliquefaciens from the Gut of Aquatic Animals. In Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB, ISBN 978-989-758-595-1, pages 855-861. DOI: 10.5220/0011297900003443

in Bibtex Style

@conference{icbeb22,
author={Hedong Lu and Chengyuan Gu and Tao Yan and Qihan Zhang and Chengxin Geng and Can Lv},
title={Screening and Identification of Bacillus amyloliquefaciens from the Gut of Aquatic Animals},
booktitle={Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,},
year={2022},
pages={855-861},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0011297900003443},
isbn={978-989-758-595-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,
TI - Screening and Identification of Bacillus amyloliquefaciens from the Gut of Aquatic Animals
SN - 978-989-758-595-1
AU - Lu H.
AU - Gu C.
AU - Yan T.
AU - Zhang Q.
AU - Geng C.
AU - Lv C.
PY - 2022
SP - 855
EP - 861
DO - 10.5220/0011297900003443