The Mechanism Study and Target Modifications on the AMP Temporin-PEa Triggering TNF-α Necroptosis Pathway in Lung Cancer Cell Death

Jiewen Zheng

2022

Abstract

NSCLC is the most typical lung cancer with one of the highest lethal rates in the world. However, the drug resistance and poor prognosis make the conventional therapies less effective. A previous study has reported that a novel AMP temporin-PEa shows anticancer activity on NSCLC, which provides more potential for lung cancer treatment. This study investigates the antiproliferation mechanism of temporin-PEa, as well as the therapeutic effect of this AMP, and two other modified versions in both in vitro and in vivo conditions. The experiments will utilize NSCLC cell lines A549 and NCI-H157, and HMEC-1 cell line, and Xenograft mice models. Antiproliferation activity is measured by MTT assay. Cytotoxicity is detected by LDH assay, and haemolytic activity is detected using horse blood. The TNF-a necroptosis will be measured by Annexin VFITC/PI assay and western blot. ROS over-generation will be measured by ROS assay kit with H2DCFDA ROS probe. The results of this study will provide important information for the exploration of the anticancer mechanism of AMPs and target modifications improving drug therapeutic values. Further studies should mainly focus on exploiting the specific target of temporin-PEa for further anticancer drug development, and in vivo drug delivery system design, which could help the drug overcome various biological barriers.

Paper Citation

in Harvard Style

Zheng J. (2022). The Mechanism Study and Target Modifications on the AMP Temporin-PEa Triggering TNF-α Necroptosis Pathway in Lung Cancer Cell Death. In Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB, ISBN 978-989-758-595-1, pages 1221-1229. DOI: 10.5220/0011390000003443

in Bibtex Style

@conference{icbeb22,
author={Jiewen Zheng},
title={The Mechanism Study and Target Modifications on the AMP Temporin-PEa Triggering TNF-α Necroptosis Pathway in Lung Cancer Cell Death},
booktitle={Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,},
year={2022},
pages={1221-1229},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0011390000003443},
isbn={978-989-758-595-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,
TI - The Mechanism Study and Target Modifications on the AMP Temporin-PEa Triggering TNF-α Necroptosis Pathway in Lung Cancer Cell Death
SN - 978-989-758-595-1
AU - Zheng J.
PY - 2022
SP - 1221
EP - 1229
DO - 10.5220/0011390000003443