Identification of MLST8, a Component of MTOR Complex 1 in Eriocheir Sinensis: CDNA Cloning and Its Expression Respond to Starvation

Zhihuan Tian, Chuanzhen Jiao

2022

Abstract

The mechanistic target of rapamycin (mTOR) signaling pathway is conserved among organisms from single-celled yeasts to complex multicellular ones such as human. Here we report the cDNA sequences and expression pattern of one gene encoding a component of mTOR complex 1 from crustacean Eriocheir sinensis, EsmLST8 (mammalian lethal with Sec13 protein 8). The deduced EsmLST8 from the cDNA sequence includes 317 amino acids (aa), which contains a WD40 superfamily region with WD40 repeats domains, forming a circularized beta-propeller structure. Expression analysis with qRT-PCR showed that in juvenile E. sinensis, the top three tissues with high mRNA levels for EsmLST8 are Y organ, stomach and hepatopancreas; the tissue with the lowest expression of mLST8 is eyestalk. After the animals’ food deprivation, the expression of EsmLST8 was significantly induced at 14d in claw muscles. These results provide basic information on the functions of mTOR signaling pathway in regulation of growth and nutritional metabolism in crustaceans.

Paper Citation

in Harvard Style

Tian Z. and Jiao C. (2022). Identification of MLST8, a Component of MTOR Complex 1 in Eriocheir Sinensis: CDNA Cloning and Its Expression Respond to Starvation. In Proceedings of the 8th International Conference on Agricultural and Biological Sciences - Volume 1: ABS, ISBN 978-989-758-607-1, pages 11-18. DOI: 10.5220/0011594400003430

in Bibtex Style

@conference{abs22,
author={Zhihuan Tian and Chuanzhen Jiao},
title={Identification of MLST8, a Component of MTOR Complex 1 in Eriocheir Sinensis: CDNA Cloning and Its Expression Respond to Starvation},
booktitle={Proceedings of the 8th International Conference on Agricultural and Biological Sciences - Volume 1: ABS,},
year={2022},
pages={11-18},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0011594400003430},
isbn={978-989-758-607-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 8th International Conference on Agricultural and Biological Sciences - Volume 1: ABS,
TI - Identification of MLST8, a Component of MTOR Complex 1 in Eriocheir Sinensis: CDNA Cloning and Its Expression Respond to Starvation
SN - 978-989-758-607-1
AU - Tian Z.
AU - Jiao C.
PY - 2022
SP - 11
EP - 18
DO - 10.5220/0011594400003430