Identifying Pulsatilla Chinesis (Bunge) Regel, and Potentilla Discolor Bunge from Each Other using Restrictive Enzyme Dde I

Erjia Wang, Shaoxuan Zhang, Dejun Sun, Guangzhu Lin, Tiantian Wang

2022

Abstract

For identifying Pulsatilla chinesis (Bunge) Regel, and Potentilla discolor Bunge from each other, a new method was established. We amplified the ITS regions of them, and sequenced the purified PCR products directly. We edited and compared the obtained sequences by Genetyx and BioEdit. The possible sites of restriction endonucleases were searched using PREMIER 5.0. It was found that Dde I can be used for their identification. In this case, we concluded that Dde I can be used effectively in identification of these plants.

Paper Citation

in Harvard Style

Wang E., Zhang S., Sun D., Lin G. and Wang T. (2022). Identifying Pulsatilla Chinesis (Bunge) Regel, and Potentilla Discolor Bunge from Each Other using Restrictive Enzyme Dde I. In Proceedings of the 8th International Conference on Agricultural and Biological Sciences - Volume 1: ABS, ISBN 978-989-758-607-1, pages 29-31. DOI: 10.5220/0011594800003430

in Bibtex Style

@conference{abs22,
author={Erjia Wang and Shaoxuan Zhang and Dejun Sun and Guangzhu Lin and Tiantian Wang},
title={Identifying Pulsatilla Chinesis (Bunge) Regel, and Potentilla Discolor Bunge from Each Other using Restrictive Enzyme Dde I},
booktitle={Proceedings of the 8th International Conference on Agricultural and Biological Sciences - Volume 1: ABS,},
year={2022},
pages={29-31},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0011594800003430},
isbn={978-989-758-607-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 8th International Conference on Agricultural and Biological Sciences - Volume 1: ABS,
TI - Identifying Pulsatilla Chinesis (Bunge) Regel, and Potentilla Discolor Bunge from Each Other using Restrictive Enzyme Dde I
SN - 978-989-758-607-1
AU - Wang E.
AU - Zhang S.
AU - Sun D.
AU - Lin G.
AU - Wang T.
PY - 2022
SP - 29
EP - 31
DO - 10.5220/0011594800003430