Evaluation of Method of Visual Gene Chip Detection for Four Pathogenic Bacteria in Aquatic Products

Lin Lu

2022

Abstract

In this manuscript, the author developed a gene chip detection method that combined tyramine signal amplification technology and nanogold-labeled silver staining technology. Four pathogenic bacteria (Listeria monocytogenes, Vibrio parahaemolyticus, V. cholera, and Staphylococcus aureus) in aquatic products were used as targets to evaluate the sensitivity, specificity and repeatability of this method. The detection sensitivity of this method can reach 103CFU/mL, and it is no different from the specificity of fluorescence detection. The coefficient of variation CV value of the hybridization repeatability results of different points on the same chip and different batches of chips were also less than 15%. The sensitivity, specificity, and reproducibility of the four visual gene chip detection methods for pathogenic bacteria in aquatic products all show good performance, which has practical promotion significance in the detection of pathogenic bacteria in aquatic products.

Paper Citation

in Harvard Style

Lu L. (2022). Evaluation of Method of Visual Gene Chip Detection for Four Pathogenic Bacteria in Aquatic Products. In Proceedings of the 8th International Conference on Agricultural and Biological Sciences - Volume 1: ABS, ISBN 978-989-758-607-1, pages 50-55. DOI: 10.5220/0011595700003430

in Bibtex Style

@conference{abs22,
author={Lin Lu},
title={Evaluation of Method of Visual Gene Chip Detection for Four Pathogenic Bacteria in Aquatic Products},
booktitle={Proceedings of the 8th International Conference on Agricultural and Biological Sciences - Volume 1: ABS,},
year={2022},
pages={50-55},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0011595700003430},
isbn={978-989-758-607-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 8th International Conference on Agricultural and Biological Sciences - Volume 1: ABS,
TI - Evaluation of Method of Visual Gene Chip Detection for Four Pathogenic Bacteria in Aquatic Products
SN - 978-989-758-607-1
AU - Lu L.
PY - 2022
SP - 50
EP - 55
DO - 10.5220/0011595700003430