Study on Methods of Protoplast Preparation of Aspergillus Niger

Xiangzhen Cui

2022

Abstract

Filamentous fungi are important industrial strains. Aspergillus niger is a safe organism for the production of proteins, organic acids, and chemicals. In order to improve the efficiency of genetic manipulation of A. niger, we studied the method of preparing protoplasts and improving the regeneration rate, and established a method of efficient transformation of protoplasts by exogenous DNA. Studies have shown that the preparation and regeneration of A. niger protoplasts are affected by many factors, such as mycelial growth state, osmotic pressure stabilizer, cell wall lyase system, enzymatic hydrolysis time, regeneration medium composition and so on. The highest efficiency of protoplast release and regeneration was obtained by using the mycelium of A. niger fresh spores cultured for 13-14 hours, using a mixture of snailase and lyticase, 0.6 M sorbitol as an osmotic pressure stabilizer, and enzymatic hydrolysis at 32 C̊ for 2 hours.

Paper Citation

in Harvard Style

Cui X. (2022). Study on Methods of Protoplast Preparation of Aspergillus Niger. In Proceedings of the 1st International Conference on Food Science and Biotechnology - Volume 1: FSB; ISBN 978-989-758-638-5, SciTePress, pages 59-62. DOI: 10.5220/0012001500003625

in Bibtex Style

@conference{fsb22,
author={Xiangzhen Cui},
title={Study on Methods of Protoplast Preparation of Aspergillus Niger},
booktitle={Proceedings of the 1st International Conference on Food Science and Biotechnology - Volume 1: FSB},
year={2022},
pages={59-62},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0012001500003625},
isbn={978-989-758-638-5},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International Conference on Food Science and Biotechnology - Volume 1: FSB
TI - Study on Methods of Protoplast Preparation of Aspergillus Niger
SN - 978-989-758-638-5
AU - Cui X.
PY - 2022
SP - 59
EP - 62
DO - 10.5220/0012001500003625
PB - SciTePress