Progress in Research Regarding Genetic Manipulation in Aspergillus Oryzae

Qi Jin, Qi Jin, Hongliang Liu, Kunhai Qin, Yitong Shang, Bin Zeng, Bin Zeng, Zhihong Hu

2022

Abstract

Aspergillus oryzae is a kind of important industrial microorganism, used in food fermentation, condiment production, brewing, recombinant protein and enzyme production. Currently, genetic engineering techniques are increasingly being used to improve fermentation performance and product yield of A. oryzae. However, unlike other filamentous fungi, such as monascus ruber or Aspergillus niger, A. oryzae has inherent resistance to the common antibiotics, which renders genetic manipulation difficult. In the past, the genetic transformation of A. oryzae has mainly relied on protoplast-mediated transformation. The Agrobacterium tumefaciens-mediated transformation system, developed in 2016, and the CRISPR/Cas9 gene editing system, has also been successfully applied in A. oryzae. In this review, we have summarized the progress in research on genetic manipulation and gene editing methods in A. oryzae.

Paper Citation

in Harvard Style

Jin Q., Liu H., Qin K., Shang Y., Zeng B. and Hu Z. (2022). Progress in Research Regarding Genetic Manipulation in Aspergillus Oryzae. In Proceedings of the 1st International Conference on Food Science and Biotechnology - Volume 1: FSB; ISBN 978-989-758-638-5, SciTePress, pages 63-69. DOI: 10.5220/0012001700003625

in Bibtex Style

@conference{fsb22,
author={Qi Jin and Hongliang Liu and Kunhai Qin and Yitong Shang and Bin Zeng and Zhihong Hu},
title={Progress in Research Regarding Genetic Manipulation in Aspergillus Oryzae},
booktitle={Proceedings of the 1st International Conference on Food Science and Biotechnology - Volume 1: FSB},
year={2022},
pages={63-69},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0012001700003625},
isbn={978-989-758-638-5},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International Conference on Food Science and Biotechnology - Volume 1: FSB
TI - Progress in Research Regarding Genetic Manipulation in Aspergillus Oryzae
SN - 978-989-758-638-5
AU - Jin Q.
AU - Liu H.
AU - Qin K.
AU - Shang Y.
AU - Zeng B.
AU - Hu Z.
PY - 2022
SP - 63
EP - 69
DO - 10.5220/0012001700003625
PB - SciTePress