Cloning and Expression Analysis of the Stable Antimicrobial Peptide Gene from Citrus Junos ’Pujang Xiangcheng’

Shu Luo, Ximeng Lin, Xiaorong Wang, Haoru Tang, Qing Chen

2022

Abstract

Citrus Huanglongbing (HLB) is a devastating disease which threatens the worldwide citrus industry. No effective curable measurement could be taken to deal with the diseases to date. The recent exciting report discovered a novel stable antimicrobial peptide (SAMP) in the HLB resistant citrus material. This SAMP could provide protective effect as well as treatment effect in citrus materials. In the present study, we cloned the CjSAMP coding gene in the newly released citrus stocks variety 'Pujiang Xiangcheng' (Citrus junos) referring to the genetic information in the C. sinensis genome. Sequence analysis revealed that the gene produced three kinds of isoforms due to alternative splicing. These three transcripts varied in length and could produce three types of proteins. Structural analysis revealed that none of these proteins could produce the effective 'coiled coil' based pore-like structure that has anti-microbial activity, indicating that 'Pujiang Xiangcheng' was not a citrus type with extreme HLB tolerance. Expression analysis was further carried out in five commonly used local stock varieties in the production application, including C. junos 'Pujiang Xiangcheng', 'Ziyang Xiangcheng', Poncirus trifoliata, citrus hybrid (P. trifoliata × Citrus sinensis) and a C. grandis. Real time quantitative PCR results indicated that C. grandis was the one with most tolerant ability, while 'Pujiang Xiangcheng' was the least tolerant one, in consistent with the protein structure prediction results.

Paper Citation

in Harvard Style

Luo S., Lin X., Wang X., Tang H. and Chen Q. (2022). Cloning and Expression Analysis of the Stable Antimicrobial Peptide Gene from Citrus Junos ’Pujang Xiangcheng’. In Proceedings of the 4th International Conference on Biotechnology and Biomedicine - Volume 1: ICBB; ISBN 978-989-758-637-8, SciTePress, pages 329-334. DOI: 10.5220/0012020600003633

in Bibtex Style

@conference{icbb22,
author={Shu Luo and Ximeng Lin and Xiaorong Wang and Haoru Tang and Qing Chen},
title={Cloning and Expression Analysis of the Stable Antimicrobial Peptide Gene from Citrus Junos ’Pujang Xiangcheng’},
booktitle={Proceedings of the 4th International Conference on Biotechnology and Biomedicine - Volume 1: ICBB},
year={2022},
pages={329-334},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0012020600003633},
isbn={978-989-758-637-8},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 4th International Conference on Biotechnology and Biomedicine - Volume 1: ICBB
TI - Cloning and Expression Analysis of the Stable Antimicrobial Peptide Gene from Citrus Junos ’Pujang Xiangcheng’
SN - 978-989-758-637-8
AU - Luo S.
AU - Lin X.
AU - Wang X.
AU - Tang H.
AU - Chen Q.
PY - 2022
SP - 329
EP - 334
DO - 10.5220/0012020600003633
PB - SciTePress