Multi-Wavelength High-Resolution Fourier Ptychographic Microscopy Using a Hemispherical LED Array

Mahdieh Mayani, Nazabat Hussain, Kim Tekseth, Dag Breiby, Dag Breiby, Muhammad Akram

2023

Abstract

Fourier Ptychographic microscopy has been proven to both increase the resolution of optical microscopes and retrieve the phase of objects using angular-varied illumination while maintaining a wide field-of-view. This work focuses on an improvement in the achieved half-pitch resolution, experimentally from 274 nm to 217 nm, by decreasing the operating wavelength from 630 nm to 470 nm. A high synthesized numerical aperture of 1.1 is obtained using 217 LEDs on a dome illuminator where the light is collected by a 10x/ 0.28NA objective lens. The experimental results closely match the theoretical prediction. As practical examples, two cartilage samples are analyzed and quantitatively imaged in this study.

Paper Citation

in Harvard Style

Mayani M., Hussain N., Tekseth K., Breiby D. and Akram M. (2023). Multi-Wavelength High-Resolution Fourier Ptychographic Microscopy Using a Hemispherical LED Array. In Proceedings of the 11th International Conference on Photonics, Optics and Laser Technology - Volume 1: PHOTOPTICS, ISBN 978-989-758-632-3, pages 17-20. DOI: 10.5220/0011594100003408

in Bibtex Style

@conference{photoptics23,
author={Mahdieh Mayani and Nazabat Hussain and Kim Tekseth and Dag Breiby and Muhammad Akram},
title={Multi-Wavelength High-Resolution Fourier Ptychographic Microscopy Using a Hemispherical LED Array},
booktitle={Proceedings of the 11th International Conference on Photonics, Optics and Laser Technology - Volume 1: PHOTOPTICS,},
year={2023},
pages={17-20},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0011594100003408},
isbn={978-989-758-632-3},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 11th International Conference on Photonics, Optics and Laser Technology - Volume 1: PHOTOPTICS,
TI - Multi-Wavelength High-Resolution Fourier Ptychographic Microscopy Using a Hemispherical LED Array
SN - 978-989-758-632-3
AU - Mayani M.
AU - Hussain N.
AU - Tekseth K.
AU - Breiby D.
AU - Akram M.
PY - 2023
SP - 17
EP - 20
DO - 10.5220/0011594100003408