In Vitro Quantification of Cellular Spheroids in Patterned Petri Dishes

Jonas Schurr, Jonas Schurr, Andreas Haghofer, Andreas Haghofer, Marian Fürsatz, Marian Fürsatz, Hannah Janout, Hannah Janout, Sylvia Nürnberger, Sylvia Nürnberger, Sylvia Nürnberger, Stephan Winkler, Stephan Winkler

2023

Abstract

Cell Spheroids are of high interest for clinical cell applications and cell screening. To allow the extraction of early readout parameters a high amount of image data of petri dishes is created. To support automated analyses of spheroids in petri dish images we present a method for analysing and quantification of spheroids in its development stages. The algorithm is based on multiple image processing algorithms and neural networks. With an evolutionary strategy, engraved grid cells on petri dish are extracted and on top a Unet is used for the segmentation and quantification of different cell compartment states. The measured f1-scores for the different states are 0.77 for monolayer grid cells, 0.86 for starting formation grid cells and 0.85 for spheroids. As we describe in this study we can provide thorough analyses of cell spheroid in petri dishes, by automating the quantification process.

Paper Citation

in Harvard Style

Schurr J., Haghofer A., Fürsatz M., Janout H., Nürnberger S. and Winkler S. (2023). In Vitro Quantification of Cellular Spheroids in Patterned Petri Dishes. In Proceedings of the 16th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2023) - Volume 2: BIOIMAGING; ISBN 978-989-758-631-6, SciTePress, pages 78-85. DOI: 10.5220/0011648700003414

in Bibtex Style

@conference{bioimaging23,
author={Jonas Schurr and Andreas Haghofer and Marian Fürsatz and Hannah Janout and Sylvia Nürnberger and Stephan Winkler},
title={In Vitro Quantification of Cellular Spheroids in Patterned Petri Dishes},
booktitle={Proceedings of the 16th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2023) - Volume 2: BIOIMAGING},
year={2023},
pages={78-85},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0011648700003414},
isbn={978-989-758-631-6},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 16th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2023) - Volume 2: BIOIMAGING
TI - In Vitro Quantification of Cellular Spheroids in Patterned Petri Dishes
SN - 978-989-758-631-6
AU - Schurr J.
AU - Haghofer A.
AU - Fürsatz M.
AU - Janout H.
AU - Nürnberger S.
AU - Winkler S.
PY - 2023
SP - 78
EP - 85
DO - 10.5220/0011648700003414
PB - SciTePress