![](bg5.png)
saturated fatty acid (SFA), 52.49 % monounsaturated
fatty acid (MUFA) and
15.66 % polyunsaturated fatty acid (PUFA) (Polat et
al., 2013).
Due to the high-lipid diet and frequent feeding,
the TG levels may be elevated all day long (Sahade et
al., 2013). Rats that were given a high-lipid diet for
30 days experienced an increase in total cholesterol,
LDL-C, TG levels and atherogenic index value, while
also showing significant decreases in the HDL-C
level (p<0.01) (Sa’adah et al., 2017). The increased
serum levels in the lipid-rich lipoproteins (LDL-C
and VLDL-C) indicate that more cholesterol and
triglyceride were transported from the liver to the
extra-hepatic tissues to be taken up by those tissues
(Adekunle et al., 2013). This process involved the
enzymes that implicate in cholesterol, lipid and
glucose metabolism. Therefore, the high-lipid diet
was presumed to increase the SREBP-1c mRNA and
protein; hence this protein appeared in the
hyperlipidemic rat groups, despite the thin protein
bands.
However, the SREBP-1c protein is actually found
in all individuals, because this protein plays a major
role in regulating the gene expression of several
enzymes implicated in cholesterol, lipid and glucose
metabolism (Dif et al., 2006).
4 CONCLUSION
The proteins that appeared in each group were
proteins with the molecular weight of 160; 144; 131;
124; 117; 110; 93; 76; 59; 52; 49; 42; 33; 25 and 14
kDa, however the protein 117 kDa was not present in
group I (control). Protein 117 kDa was presumed as
the sterol regulatory element binding protein-1c (His-
SREBP-1c), the transcription factor that transduces
the insulin signal. A high-lipid diet was presumed to
increase the SREBP-1c mRNA and protein, so this
protein appeared in the hyperlipidemic rat groups,
despite the thin protein bands.
ACKNOWLEDGEMENTS
The authors would like to express their gratitude
to Institut Teknologi Sepuluh Nopember (ITS)
Surabaya for the financial support. This research was
a grant for novice lecturers and was funded by ITS;
while the Zoology and Animal Engineering
Laboratory, Biology Department, ITS Surabaya; the
Experimental Animal Laboratory, Faculty of
Pharmacy, Airlangga University; and the
Biochemistry Laboratory, Pusat Antar Universitas
(PAU) Universitas Gadjah Mada (UGM) provided
materials and technical assistance.
REFERENCES
A. S. Adekunle, A. L. Adedeji, E. O. Oyewo, et al., Asian
Journal of Natural & Applied Sciences 2(1), 82–93
(2013).http://www.ajsc.leena-
luna.co.jp/AJSCPDFs/Vol.2(1)/AJSC2013(2.1−11).
pdf
Botolin D and Jump. DB. 2003. Selective proteolytic
processing of rat hepatic sterol regulatory element
binding protein-1 (SREBP-1) and SREBP-2 during
postnatal development.J Biol Chem. 2003 Feb
28;278(9):6959-
62.https://www.ncbi.nlm.nih.gov/pubmed/12488438
E. S. Polat, O. B. Citil, and M. Garip. 2013. Fatty acid
composition of yolk of nine poultry species kept in
their natural environment. Animal Science Papers
and Reports 31(4), 363–368 (2013).
http://agro.icm.edu.pl/agro/element/bwmeta1.elemen
t.agro-a8f6b877-4fe0-4886-b23c-ef77928fb2fe/
F. A.Khesht and A.Hassanabadi. 2012. Effects of sterol
regulatory element-bindingprotein (SREBP) in
chickens. Lipids in Health and Disease 2012, 11:20.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC330
5589/pdf/1476-511X-11-20.pdf
F.Hansmannel, S.Mordier and P. B. Iynedjian. 2006.
Insulin induction of glucokinase and fatty acid
synthase in hepatocytes:analysis of the roles of sterol-
regulatory-element-binding protein-1c andliver X
receptor. Biochem. J. (2006) 399, 275–283.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC160
9914/pdf/bj3990275.pdf
F. Martins, T. M. Noso, V. B. Porto, et al. 2010. Maté tea
inhibits in vitro pancreatic lipase activity and has
hypolipidemic effect on high-fat diet-induced obese
mice. Obesity (Silver Spring) 18 (1), 42–47.
https://www.ncbi.nlm.nih.gov/pubmed/19543216
Foufelle, F., and P. Ferre. P. 2002. New perspectives in the
regulation of hepaticglycolytic and lipogenic genes
by insulin and glucose: a role for the transcription
factor sterolregulatory element binding protein-1c.
Biochem. J. 366, 377-
391.https://www.ncbi.nlm.nih.gov/pmc/articles/PM
C1222807/pdf/12061893.pdf
Gosmain, Y., N. Dif, V. Berbe, E. Loizon, J. Rieusset, H.
Vidal, and E. Lefai. 2005. Regulation of SREBP-1
expression and transcriptional action on HKII and
FAS genes duringfasting and refeeding in rat tissues.
J. Lipid. Res. 46, 697-705.
https://www.ncbi.nlm.nih.gov/pubmed/15627654
I. Tusanti, A. Johan, and R. A. Kisdjamiatun. 2014.
Sitotoksisitas in vitro ekstraketanolikbuahparijoto
(Medinilla speciosa, reinw.ex bl.)
terhadapselkankerpayudara T47D. JurnalGizi
Antihiperlipidemic Activity of the Methanolic Extract of Parijoto (Medinilla speciosa) on the Protein Profile of Hyperlipidemic Rats
127