therapies available for S. aureus infection. In 2003, a
new strain of MRSA caused outbreak of cutaneous
infection and pneumonia.
Compared to other resistant bacteria, MRSA
infection is epidemiologically significant. Studies
conducted by CDC more than half Staphylococci
bacteria ehich caused Hospital Acquired Infections
are resistant to oxacillin (De Angelis G et al, 2010)
and its infection nowadays is an endemic in US
hospitals and communities (Klein E et al, 2007;
Crum NFet al, 2006). Furthermore, MRSA in Cipto
Mangukusumo Hospital, Indonesia also show an
increase from 28.5% in 2009 to 32% in 2010 (Liana
P., 2014).
Several research to find a novel anti
Staphyococcal bacteria from plants is also
progressing. Pradhan D et al, 2013 and Dwivedi Vet
al, 2014 reported Piper betle leaves and leaves
extract have antimicrobial, anti-inflammatory,
antioxidant and antiseptic properties. Specifically, P.
betle shown to have antibacterial activity against S.
aureus, Streptococcus pyogenes, E. coli,
Pseudomonas aeruginosa, Enterococcus fecalis,
Klebsiella pneumoniae, and others. The content of
sterols in betel leaf extract interact with bacterial cell
wall, disturbing its permeability.
Other plant that also potential to be an
antibacterial are bark of candlenut (Aleurites
moluccana) which is used traditionally for the
treatment of diarrhea and thypoid fever
(Alimboyoguen AB, et al 2014). Research shows
3acetyl aleuritolic acid from bark extract has an
antimicrobial activity. Moluccanin from A.
moluccana also has antibacterial including S. aureus
and antiviral activity (Othman AS and Rasyidah
MR, 2010).
Albizia saman (Jacq.) Merr. formerly known as
Samanea saman is having several phytochemical
components which are flavonoids, alkaloids, tannins,
carbohydrates, glycosides, saponins, steroids, and
reducing sugar are widely used as the remedy for
colds, diarrhea, headache, and stomach ache.
According to Perry in 1980, the alcoholic extract of
S. saman is also proven to inhibit the growth of
Mycobacterium tuberculosis (Kirithika T.2013).
The clove plant (Syzygium aromaticum) contain
chemical compounds that provide its aromatic and
antibacterial nature. The active compound being
studied is eugenol, one of many phenolic
compounds. Eugenol has been widely used in dental
care settings, and has been proven as an effective
anesthetic and antiseptic (Cortés-Rojas Det al, 2016;
Neveu Vet al, 2010).
With its promising properties as antibacterial and
its abundancy worldwide, we assessed several
concentration of P. betle leaves extract, A.
moluccana stem bark extract, S. saman extract, C.
longa extract and S. aromaticum flower bud extract
against S. epidermidis, S. aureus and MRSA. As our
result show that several extracts have a good
potency as anti-staphylococcal infection.
2 MATERIAL AND METHODS
2.1 Bacteria, Medium and Extract
S. epidermidis, S. aureus and MRSA bacteria were
grown in nutrient agar. All bacteria were from
Microbiology Department culture collection, Faculty
of Medicine Universitas Indonesia, which identified
using commercial Vitex identification kit and tested
for its resistancy according to CLSI. Broth Brain
Heart Infusion (BHI) medium and Muller Hinton
Agar (MHA) for antibacterial testing and Plate
Count Agar (PCA) were provided by Department of
Microbiology, Faculty of Medicine, Universitas
Indonesia. Extracts of P. betle leaves, A. moluccana
stem bark, S. saman, C.longa and S. aromaticum
flower bud in ethanol were prepared by Medical
Pharmacy Department, Faculty of Medicine,
Universitas Indonesia. Antibiotic ciprofloxacin or
clindamycin was used for positive control.
2.2 Antibacterial Assay
2.2.1 Agar diffusion method
An overnight bacteria culture was diluted into 0.9%
NaCl to reach McFarland value of 0.5. Bacterial
suspension was then applied into MHA followed by
creating 7 diffusion wells in the media using blue
tips. Each extract at several concentrations was
applied in to the well which are : P.betle extract at
62,5; 125; 250; 500 and 1000 mg/ml was tested
against S. epidermidis and A.moluccana extract at
50; 100; 200; 400 and 800 µg/ml against MRSA.
With addition for aquadest and antibiotics at 20
µg/ml as negative and positive control respectively.
Plate was then incubated for 16 – 18 hours at 37
o
C.
Observed inhibitory zone was measured using
calipers.
BROMO 2018 - Bromo Conference, Symposium on Natural Products and Biodiversity
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