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Fig. 1 Effect of Diterpene Lactone Fraction of
“Sambiloto” (A. paniculata Nees.) on Glucose Level
The result showed that positive control treated
with glibenclamide suspension was obtained an
average reduction in blood glucose levels of 263 ±
14.3 (44.1%). Negative control, giving a suspension
0.5% CMC Na, didn’t give effect in decreasing
blood glucose levels of -18.7 ± 13.7 (-3.64%) and it
become a baseline of this experiment. Based on
SPSS statistical analysis, it showed that positive
control had a significant difference to negative
control with p calculated value of 0.000 (p <0.05). It
indicated that positive control was able to lower
blood glucose levels significantly over an 24-hour
and antidiabetic test method used in this study was
correct.
The first treatment group that containing 7.4
mg/20 g BW diterpene lactone fraction resulted in
an average reduction in blood glucose levels of 59.7
± 18.2 (14.8%). It had p calculated value at 0.013 (p
<0.05) when compared to negative control using
statistical analysis Anova One Way and Post Hoc
LSD. It showed that group 1 was able to lower blood
glucose levels significantly.
The second treatment group that containing 4.9
mg/20 g BW diterpene lactone fraction resulted in
an average reduction in blood glucose levels of 144
± 34.6 (35.9%). Based on statistical analysis Anova
One Way and Post Hoc LSD result, it had p
calculated value at 0.000 (p <0.05) when compared
to negative control. It showed that group 2 was able
to lower blood glucose levels significantly.
The third treatment group that containing 22.3
mg/20 g BW diterpene lactone fraction resulted in
an average reduction in blood glucose levels of 111
± 15.1 (27.6%). Based on statistical analysis Anova
One Way and Post Hoc LSD, it had p calculated
value at 0.000 (p <0.05) when compared to negative
control. It showed that group 3 was able to lower
blood glucose levels significantly.
Comparising data between groups 1 and 2 was
obtained significantly different with a significance
value of 0.008 (p <0.05), whereas between group 1
and 3 and group 2 and 3 were no significant
differences with a significance value of 0.095 (p>
0.05) and 0.260 (p <0.05), respectively. It can be
concluded that group 2 was the most effective dose
for lowering blood glucose levels.
Comparising data between positive control and
three treatment groups to negative control was
obtained no significant difference (p count <0.05),
which means positive control and three treatment
groups can lower blood glucose levels in mice
significantly for 24 hours. Then three treatment
groups compared to positive control showed
significant difference (p count <0.05) and it had
greater average value of a decreasing blood glucose
levels than positive control. It can be concluded that
the fraction of diterpene lactone (group 1, 2 and 3)
had antidiabetic activity but their decreasing blood
glucose levels were not as big as a positive control.
Thus, the hypothesis in this study accepted that
diterpene lactone fraction can decrease blood
glucose levels. Compound suspected which has
activity lowers blood glucose levels is
andrographolide with triggering mechanism
secretion of insulin so glucose metabolism increases,
it can cause blood glucose levels to decline [7].
The results of this study can be used as scientific
information and supporting data for further studies
in order to develop diterpene lactone fraction as
dosage forms of antidiabetic phytopharmaceutical
drugs or raw materials. It had been found the
effective dose of diterpene lactone fraction that can
be used as traditional or complementary drug
candidate for antidiabetic drugs is safe. Furthermore,
it can be done praformulation study (physical
characteristics, physicochemical characteristics,
biofarmasetika characteristics, compatibility, and
stability) and formulations diterpene lactone fraction
from “sambiloto”.
4 CONCLUSION
Diterpene lactone fraction of “Sambiloto” at 7.4
mg/20 g body weight, 14.9 mg / 20 g body weight,
and 22.3 mg / 20 g body weight had antidiabetic
activity in mice induced by alloxan. The most
effective dose was 14.9 mg/20 g body weight.
Antidiabetic Activity of Diterpene Lactone Fraction of "Sambiloto" (Andrographis paniculata Nees.) on Mice (Mus musculus) Induced by
Alloxan
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