Standardization Raw Material and Ethanolic Extract of
Andrographidis Herba (Andrographis Paniculata Nees) from District
of Bogor and Tawangmangu
Sukardiman, Rakhmawati, Alvi Prabowo , Lusiana Arifianti
Department of Pharmacognosy and phytochemical, Faculty of Pharmacy, Airlangga University, Surabaya, Indonesia
Keyword: Standardization, raw material, ethanolic extract, Andrographis paniculata Nees, Andrographis herbs
Abstract: Sambiloto (Andrographis paniculata Nees) has a potency to be developed in phyto-pharmaceutical product,
especially in this study focused on supporting anti-hyperglycemic research. This requires standardization
can affect both the raw material or extract qualities that have been obtained based on the Indonesian Herbal
Pharmacopeia method. Raw material for this study has been obtained from two traditional drug
development institutions, there are BALITRO from Bogor and BPTO from Tawangmangu
1 INTRODUCTION
Sambiloto (Andrographis paniculata Nees) is one of
medicinal plant that it has long existed and used as
ingredient of traditional medicine. Sambiloto has
bitter taste, contains flavonoids, tannins, and
saponins [1].
Ethanolic extract of sambiloto can restore blood
glucose level into normal in induced-rats, it
indicated as anti-hyperglicemic activity [2]. Another
study showed that combination curcumin and
andrographolide (3:1) have role in reducing blood
glucose level in rats by 47.945% was preprandial
and 74.159% was postprandial [3]. The study of
sambiloto as anti-hyperglicemic also showed in the
mixture formula such as sambiloto and mahoni
reduced blood glucose level in mice by 250.80 mg/dl
[4].
Many studies have proven the efficacy of
sambiloto as anti-diabetic agent and raises potential
to be developed into phyto-pharmaceutical product
as anti-diabetic. Therefore, it is necessary to do
standardization. It can affect quality of both extract
and raw material by methods that have been
established by Ministry of Health through Indonesia
Herbal Pharmacopeia. It is a guarantee for safety,
effectiveness, and especially quality of plant
material, raw material and ethanolic extract that
developed into phyto-pharmaceutical preparation.
2 MATERIAL AND METHODS
2.1. Plant Material
In this research, the raw material was obtained from
two different districts, there are Bogor (BALITRO,
Balai Penelitian Tanaman Obat dan Aromatik) and
Tawangmangu (BPTO, Balai Besar Penelitian dan
Pengembangan Tanaman Obat dan Obat
Tradisional). They prepared in dried raw material.
The ethanolic extract was extracted by maceration
method with 96% ethanol for 24 hours and
performed up to three times [5]. The mixture extract
was concentrate using rotary evaporator.
2.2. Determination of Specific and Non-
specific Parameters of Dried Raw
Material and Ethanolic Extract of
Sambiloto
Determination of standard parameter was done using
Ministry of Health Guidelines through Indonesian
Herbal Pharmacopeia [6]. Specific parameters of
raw material and extract were macroscopic,
macroscopic, organoleptic, water-soluble extractive,
ethanol soluble extractive, and andrographolide
assay in raw material as well as ethanolic extract.
While non-specific parameters encompassed loss on
Sukardiman, ., Rakhmawati, ., Prabowo, A. and Arifianti, L.
Standardization Raw Material and Ethanolic Extract of Andrographidis Herba (Andrographis paniculata Nees) from District of Bogor and Tawangmangu.
DOI: 10.5220/0009846000002406
In Proceedings of BROMO Conference (BROMO 2018) - Symposium on Natural Product and Biodiversity, page 1
ISBN: 978-989-758-347-6
Copyright
c
2022 by SCITEPRESS – Science and Technology Publications, Lda. All rights reserved
1