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Figure 3. Cyclin D1 analysis using flow cytometry. T47D cells were treated by nHF for 24h and stained using cyclin D1
antibody. (a) control cells unstaining; (b) control cells; (c) nHF 35 µg/mL.
Geraniol is one of monoterpene which has
suppressed the MCF-7 growth through induction cell
cycle arrest in G
1
phase, reduce the level of cyclin
D1, cyclin dependent kinase 4 (CDK4), cyclin E and
cyclin A (Duncan, et al., 2004).
Based on the results above, we conclude that n-
hexane fraction of Cyperus rotundus L. rhizome has
cytotoxic activity towards T47D breast cancer cells
which have some mechanism such as induction of
apoptosis and inhibition of cell cycle especially in
inhibit cyclin D1 expression.
ACKNOWLEDGEMENT
We gratefully thank to Research Center University
of Sumatera Utara through “Hibah Penelitian Guru
Besar” Research Grant 2018 “No:2590/
UN5.1.R/PPM/2018” for financial support in the
study.
REFERENCES
Anggraeni R, Hadisahputra S, Silalahi J, Satria D (2014).
Combinational effects of ethylacetate extract of
Zanthoxylum acanthopodium DC. with doxorubicin on
T47D breast cancer cells. Int J PharmTech Res, 6,
2032-5.
Crowell PL (1999). Prevention and therapy of cancer by
dietary monoterpenes. J Nutr 129:775S–778S.
Dalimunthe A, Hasibuan PAZ, Satria D. (2017). Cell cycle
arrest activity of Litsea cubeba Lout. heartwood and
fruit extracts against T47D breast cancer cells. Asian J
Pharm Clin Resc, 10(11), 404-6.
Dalimunthe A, Hasibuan PAZ, Silalahi J, Sinaga SF,
Satria D. (2018). Antioxidant activity of alkaloid
compounds from Litsea cubeba Lour. Orient J Chem,
34(2), 1149-52.
Duncan RE, Lau D, El-Sohemy A and Archer MC. (2004).
Geraniol and beta-ionone inhibit proliferation, cell
cycle progression, and cyclin-dependent kinase 2
activity in MCF-7 breast cancer cells independent of
effects on HMG-CoA reductase activity. Biochem
Pharmacol 68: 1739-1747.
Elson CE and Yu SG (1994) The chemoprevention of
cancer by mevalonate-derived constituents of fruits
and vegetables. J Nutr 124:607–614.
Hadi SM, Bhat SH, Azmi AS, Hanif S, ShamimU, Ullah
MF. (2007). Oxidative breakage of cellular DNA by
plant polyphenols: a putative mechanism for
anticancer properties. Semin Cancer Biol. 17, 370–6.
Handayani S, Susidarti RA, Jenie RI, Meiyanto E. (2017).
Two active compounds from Caesalpinia sappan L. in
combination with cisplatin synergistically induce
apoptosis and cell cycle arrest on WiDr cells. Adv
Pharm Bull, 7(3), 375-80.
Harahap U, Hasibuan PAZ, Sitorus P, Arfian N, Satria D.
(2018). Antimigration activity of an ethylacetate
fraction of Zanthoxylum acanthopodium DC. fruits in
4T1 breast cancer cells. Asian Pac J Cancer Prev,
19(2), 565-9.
Hasibuan PAZ, Jessy C, Denny S (2015). Combination
effect of ethylacetate extracts of Plectranthus
ambonicius (Lour.) Spreng. with doxorubicin againts
T47D breast cancer cells. Int J Pharm Pharm Sci, 7,
155-9.
Hemanth K, Razack S, Nallamuthu I, Khanum F. (2014)
Phytochemical analysis and biological properties of
Cyperus rotundus L. Ind. Crop Prod. 52, 815–26.
Jirovetz L, Wobus A, Buchbauer G, Shafi MP, Thampi
PT. (2004). Comparative analysis of the essential oil
and SPME-headspace aroma compounds of Cyperus
rotundus L. roots/tubers from South-India using GC,
GC-MS and olfactometry. J. Essent. Oil-Bearing
Plants. 7, 100-6.
Kelloff GJ, Boone CW, Crowell JA, Steele VE, Lubet RA,
Doody LA, Malone WF, Hawk ET and Sigman CC
(1996) New agents for cancer chemoprevention. J Cell
Biochem 26:1–28.
Kilani, S. (2007). Chemical investigation of different
extracts and essential oil from the tubers of (Tunisian)
Cyperus rotundus. Correlation with their antiradical
and antimutagenic properties. Ann. Microbiol. 57,
657–64.
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