Chemical Constituents and Comprehensive Application of the
Essential Oil from Artemisia Argyi
Bukun Jin
1
, Hongwei Zhai
1
, Na Qu
2
, Hui Hu
1,*
, Lin Long
1
, Haihua Huang
1
, Qingan Li
1
and Daihua Sun
1
1
Jing Brand Chi Zhengtang Pharmaceutical Co., Ltd, Huangshi 435100, China
2
Jing Brand Research Institute, Jing Brand Co., Ltd., Daye 435100, China
Keywords: Artemisia Argyi, Essential Oil, Constituents, Antibacterial Activity.
Abstract: Objective: To research the chemical constituents of antibacterial active ingredient of the essential oil from the
artemisia argyi. Methods: High performance liquid chromatography and gas chromatography-mass
spectrometry were applied for the identification of chemical constituents in different extraction methods from
Artemisia argyi. Results: Fifty chemical components were detected and twenty compounds were identified in
the essential oil. The chemical constituents were mainly including artemisol (55.49%), 3,3,6-Trimethyl-1, 4-
heptadien-6-ol (15.37%), Eucalyptol (11.87%) and 4-methyl-1-(1-methylethyl)- 3-Cyclohexen-1-ol (3.50%),
mostly composed of terpenoids. Conclusion: The main components in the essential oil and water exraction
from artemisia argyi were elucidated.
1 INTRODUCTION
The essential oils of traditional chinese medicine are
present in various aromatic plants such as artemisiae
argyi, amomi fructus, cinnamomi cortex, zanthoxyli
pericarpium et al. Several techniques have been used
to obtain essential oils from the plant, including steam
distillation, supercritical fluid extraction, solvent
extraction. Yuan Haibin analyzed the volatile oil
extraction rate in solvent extraction and supercritical-
CO
2
fluid extraction, and compared the main
components. The extraction rate was 2.7 times higer
than solvent extraction through supercritical-CO
2
fluid extraction, and thirty-one compositions were
defined by solvent extraction and its main
compositions were 2,5,5-trimethyl-1,3,6-Heptatriene,
β-Pinene and et al. Twenty-two compositions were
defined by supercritical-CO
2
fluid extraction.
Therefore, we selected the asolvent extraction for
further research. The main compositions of essential
oils are low molecular weight aroma chemicals such
as aromatic and aliphatic compounds. Now, essential
oils and some of their extract components from
traditional Chinese medicine have been widely used
*
Corresponding Author
in air fresheners, food additives, cosmetics, as well as
in medicinal uses (Aparicio-Soto 2016).
Artemisia argyi is traditional Chinese herbal
medicinewidely which is scattered throughout China,
and frequently used for diseases treatment including
inflammation, eczema and tuberculosis (Gao 2017).
Recently study on Artemisia argyi found that both its
volatile oil and water extracts exhibited good
antibacterial, immune regulation effects, anti-
inflammatory (Chen 2017). In-depth studies on the
chemical constituents of artemisia argyi have shown
that many constituents are identified from artemisia
argyi extract and dried leaves, such as 1,8-cineole,
borneol oil, chlorogenic acid, neochlorogenic acid,
cryptochlorogenic acid, Isochlorogenic acid b,
isochlorogenic acid c and pigenin (Dai 2015). The
main purpose of this paper was to comparative study
on volatile oil components and water extract
components of argyi leaves and further identify
antimicrobial components on the basis of the
Preparation of the Essential Oil from Artemisia Argyi
Grown in Qichun, China and its application in
Antibacterial effection.
400
Jin, B., Zhai, H., Qu, N., Hu, H., Long, L., Huang, H., Li, Q. and Sun, D.
Chemical Constituents and Comprehensive Application of the Essential Oil from Artemisia Argyi.
DOI: 10.5220/0012021700003633
In Proceedings of the 4th International Conference on Biotechnology and Biomedicine (ICBB 2022), pages 400-403
ISBN: 978-989-758-637-8
Copyright
c
 2023 by SCITEPRESS – Science and Technology Publications, Lda. Under CC license (CC BY-NC-ND 4.0)

2 MATERIALS AND METHODS
2.1 Material and Essential Oil
Extraction
Artemisia argyi were collected during May in China.
Five samples of the leaves (5kg for each samples)
from artemisia argyi were hydrodistilled for 5 h in
extraction equipment. The essential oil and water
extraction were collected for further analysis (Li
2021).
2.2 High Performance Liquid
Chromatography
Fingerprints of water extraction of artemisia argyi
were determined by HPLC-PDA. All samples were
analyzed by HSS T3 C18 (150mm×2.1mm, 1.8μm)
maintained at 30℃, and eluted with acetonitrile-0.1%
formic acid at the flow rate of 0.3mL/min, and the
detection wavelength was 235nm respectively (Shin
2017).
Peak 1: Neochlorogenic acid; Peak 2: Chlorogenic acid; Peak 3: Cryptochlorogenic acid; Peak 4: Isochlorogenic Acid B;
Peak 5: Isochlorogenic acid C
Figure 1: Fingerprint of artemisia argyi.
2.3 Gas Chromatography-Mass
Spectrometry (GC-MS)
GC-MS analyses were performed on an Agilent
Technologies 6890 Plus equipped with an Agilent
Technologies MSD 5973 mass-selective detector and
the same columns (Seo 2003). Helium was the carrier
gas at a flow rate of 1.5 mL/min. Column temperature
was initially at 60°C for 5 min and then gradually
increased to 200°C at a 5°C/min rate and held for 20
min. Injector temperature was set at 250°C (Silva
2003). For mass spectrometry detection. The injector,
ion source and quadrupole rod temperatures were set
at 250°C, 230°C and 150°C, respectively. The
injection volume was 10 μL with split ratio 1:20.
Mass spectrum recorded under electron impact
ionization mode (EI) with ionization energy 70 eV
and mass scan range of 55–550 amu (Tsibranska
2003).
Figure 2: Total ion chromatogram the volatile oil of artemisia argyi.
Time[min]
Signal[mv]
Chemical Constituents and Comprehensive Application of the Essential Oil from Artemisia Argyi
401

Table 1: Composition of the essential oil from artemisia argyi grown in Qichun.
No.
Retention
time / min
Chemical
formula
Molecular
weight
Compounds
Relative
content
/%
1 8.166
C
10
H
16
136 α-Pinene
0.31%
2 9.149 C
10
H
16
136
2,5,5-trimethyl-1,3,6-Heptatriene
0.22%
3 10.281
C
10
H
16
136 β-Pinene (Tautenhahn 2012)
0.08%
4 12.301 C
10
H
16
136
1-methyl-4-(1-methylethyl)- 1,3-
C
y
clohexadiene
0.38%
5 12.861
C
10
H
16
136 Limonene
0.14%
6 13.171
C
10
H
18
O 154 Eucalyptol
11.87%
7 14.241 C
10
H
16
136
1-methyl-4-(1-methylethyl)- 1,4-
C
y
clohexadiene
0.70%
8 15.028 C
10
H
14
134
1-methyl-2-(1-methylethyl)-Benzene
0.88%
9 17.209 C
3
H
5
N
3
83
3-aminopyrazole
0.63%
10 18.408 C
10
H
18
O 154
3,3,6-Trimethyl-1,4-heptadien-6-ol
15.37%
11 19.787 C
8
H
16
O 128
1-octen-3-ol (Xiao 2018)
0.34%
12 20.266 C
10
H
18
O 154
terpineol
0.59%
13 21.442 C
10
H
18
O 154
3,3,6-Trimethyl-1,5-heptadien-4-ol
55.49%
14 23.89 C
10
H
18
O 154
4-methyl-1-(1-methylethyl)- 3-
C
y
clohexen-1-ol
3.50%
15 26.06 C
10
H
16
136
4-carene (Zhang 2013)
1.23%
16 27.209 C
10
H
16
O 152
2-methyl-5-(1-methylethyl)- 2-
C
y
clohexen-1-one
0.10%
17 28.379 C
10
H
16
O 152
4-(1-methylethyl)- 1-Cyclohexen-1-
carboxaldeh
y
de
0.30%
18 29.104 C
10
H
16
O 152
Carveol
1.43%
19 32.491 C
15
H
24
O 220
Caryophyllene oxide
1.80%
20 36.218 C
10
H
12
O
2
164
Eugenol (Zhang 2010)
0.59%
3 CONCLUSION
In this study the antibacterial activity and chemical
composition of the essential oil from artemisia argyi
grown in Qichun (China) was analyzed. Among the
twenty compounds identified, terpenoids were the
most represented classes of volatiles which possessed
93.51%. Monoterpenes, which include monoterpene
hydrocarbons and oxygenated monoterpenes, were
the predominant chemical class of essential oil
constituents. Most of the high content compositions
such as artemisol (55.49%), 3,3,6-Trimethyl-1,4-
heptadien-6-ol (15.37%), Eucalyptol (11.87%), 4-
methyl-1-(1-methylethyl)-3-Cyclohexen-1-ol
(3.50%), Caryophyllene oxide (1.80%) and Carveol
(1.43%) identified in this study were belonged to
oxygenated monoterpenes. Overall, artemisol
(55.49%) had the highest concentration compared
with other constituents in the essential oil from the
artemisia argyi grown in Qichun (China). Presently,
the highest content of artemisol identified in the
volatile oil component was 67.83%, which is from
seriphidium mongolorum. However, in the past report
the volatile oil from artemisia argyi had a low content
of artemisol or even no detected. Therefore, this study
was the first time to obtain highest content of
artemisol from artemisia argyi. This result might be
attributed to the special geographical environment in
Qichun (China). The literature on the
pharmacological effects of artemisol is rarely
reported, and its application value needs further
research.
ACKNOWLEDGEMENT
This research was financially supported by Hubei
Province Technology Innovation Special Project
(NO. 2020ACA007-03).
ICBB 2022 - International Conference on Biotechnology and Biomedicine
402

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