ESTIMATING LARGE LOCAL MOTION IN LIVE-CELL IMAGING USING VARIATIONAL OPTICAL FLOW - Towards Motion Tracking in Live Cell Imaging Using Optical Flow

Jan Hubený, Vladimír Ulman, Pavel Matula

2007

Abstract

The paper studies the application of state-of-the-art variational optical flow methods for motion tracking of fluorescently labeled targets in living cells. Four variants of variational optical flow methods suitable for this task are briefly described and evaluated in terms of the average angular error. Artificial ground-truth image sequences were generated for the purpose of this evaluation. The aim was to compare the ability of those methods to estimate local divergent motion and their suitability for data with combined global and local motion. Parametric studies were performed in order to find the most suitable parameter adjustment. It is shown that a selected optimally tuned method tested on real 3D input data produced satisfactory results. Finally, it is shown that by using appropriate numerical solution, reasonable computational times can be achieved even for 3D image sequences.

Paper Citation

in Harvard Style

Hubený J., Ulman V. and Matula P. (2007). ESTIMATING LARGE LOCAL MOTION IN LIVE-CELL IMAGING USING VARIATIONAL OPTICAL FLOW - Towards Motion Tracking in Live Cell Imaging Using Optical Flow . In Proceedings of the Second International Conference on Computer Vision Theory and Applications - Volume 2: VISAPP, ISBN 978-972-8865-74-0, pages 542-548. DOI: 10.5220/0002056405420548

in Bibtex Style

@conference{visapp07,
author={Jan Hubený and Vladimír Ulman and Pavel Matula},
title={ESTIMATING LARGE LOCAL MOTION IN LIVE-CELL IMAGING USING VARIATIONAL OPTICAL FLOW - Towards Motion Tracking in Live Cell Imaging Using Optical Flow},
booktitle={Proceedings of the Second International Conference on Computer Vision Theory and Applications - Volume 2: VISAPP,},
year={2007},
pages={542-548},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0002056405420548},
isbn={978-972-8865-74-0},
}

in EndNote Style

TY - CONF
JO - Proceedings of the Second International Conference on Computer Vision Theory and Applications - Volume 2: VISAPP,
TI - ESTIMATING LARGE LOCAL MOTION IN LIVE-CELL IMAGING USING VARIATIONAL OPTICAL FLOW - Towards Motion Tracking in Live Cell Imaging Using Optical Flow
SN - 978-972-8865-74-0
AU - Hubený J.
AU - Ulman V.
AU - Matula P.
PY - 2007
SP - 542
EP - 548
DO - 10.5220/0002056405420548