FEASABILITY OF YEAST AND BACTERIA IDENTIFICATION USING UV-VIS-SWNIR DIFUSIVE REFLECTANCE SPECTROSCOPY

J. S. Silva, R. C. Martins, A. A. Vicente, J. A. Teixeira

2008

Abstract

UV-VIS spectroscopy is a powerfull qualitative and quantitative technique used in analytical chemistry, which gives information about electronic transitions of electrons in molecular orbitals. As in UV-VIS spectra there is no direct information on characteristic organic groups, vibrational spectroscopy (e.g. infrared) has been preferred for biological applications. In this research, we try to use state-of-the-art fiber optics probes to obtain UV-VIS-SWNIR diffusive reflectance measurements of yeasts and bacteria colonies on plate count agar in the region of 200-1200nm; in order to discriminate the following microorganisms: i) yeasts: Saccharomyces cerevisiae, Saccharomyces bayanus, Candida albicans, Yarrowia lipolytica; and ii) bacteria: Micrococcus luteus, Pseudomonas fluorescens, Escherichia coli, Bacillus cereus. Spectroscopy results show that UV-VIS-SWNIR has great potential for identifying microorganisms on plate count agar. Scattering artifacts of both colonies and plate count agar can be significantly removed using a robust mean scattering algorithm, allowing also better discriminations between the scores obtained by singular value decomposition. Hierarchical clustering analysis of UV-VIS and VIS-SWNIR decomposed spectral scores lead to the conclusion that the use of VIS-SWNIR light source produces higher discrimination ratios for all the studied microorganisms, presenting great potential for developing biotechnology applications.

Paper Citation

in Harvard Style

S. Silva J., C. Martins R., A. Vicente A. and A. Teixeira J. (2008). FEASABILITY OF YEAST AND BACTERIA IDENTIFICATION USING UV-VIS-SWNIR DIFUSIVE REFLECTANCE SPECTROSCOPY . In Proceedings of the First International Conference on Bio-inspired Systems and Signal Processing - Volume 1: BIOSIGNALS, (BIOSTEC 2008) ISBN 978-989-8111-18-0, pages 25-32. DOI: 10.5220/0001062800250032

in Bibtex Style

@conference{biosignals08,
author={J. S. Silva and R. C. Martins and A. A. Vicente and J. A. Teixeira},
title={FEASABILITY OF YEAST AND BACTERIA IDENTIFICATION USING UV-VIS-SWNIR DIFUSIVE REFLECTANCE SPECTROSCOPY},
booktitle={Proceedings of the First International Conference on Bio-inspired Systems and Signal Processing - Volume 1: BIOSIGNALS, (BIOSTEC 2008)},
year={2008},
pages={25-32},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0001062800250032},
isbn={978-989-8111-18-0},
}

in EndNote Style

TY - CONF
JO - Proceedings of the First International Conference on Bio-inspired Systems and Signal Processing - Volume 1: BIOSIGNALS, (BIOSTEC 2008)
TI - FEASABILITY OF YEAST AND BACTERIA IDENTIFICATION USING UV-VIS-SWNIR DIFUSIVE REFLECTANCE SPECTROSCOPY
SN - 978-989-8111-18-0
AU - S. Silva J.
AU - C. Martins R.
AU - A. Vicente A.
AU - A. Teixeira J.
PY - 2008
SP - 25
EP - 32
DO - 10.5220/0001062800250032