OPTIMIZATION OF ELECTRIC FIELD FREQUENCY ON DIELECTROPHORETIC IMPEDANCE MEASUREMENT METHOD FOR ORAL BACTERIA DETECTION

Ryo Hamada, Junya Suehiro

2011

Abstract

A simple and rapid bacteria detection device for on-site evaluation of oral hygiene in hospitals and clinics was demonstrated. The developed device utilizes dielectrophoretic impedance measurement (DEPIM) method. We integrated a micro electrode chip on which bacteria were captured by dielectrophoresis (DEP), an AC voltage source to induce DEP force, and an impedance measurement circuit to a portable instrument that enables rapid and automated oral bacterial inspection in hospitals and clinics. Special considerations have been made on effects of high electrical conductivity of oral samples on DEP force and DEPIM results. It was shown experimentally and theoretically that using a higher electric field frequency for the DEP bacteria trap and the impedance measurement could realize DEPIM application to bacteria inspection from oral samples with higher conductivity. Based on these investigations, we optimized the frequency condition of the DEPIM suitable for inspecting an oral sample along with the design and development of a portable DEPIM apparatus for on-site inspection of oral bacteria.

References

  1. Abe S., Ishihara K., Adachi M., Sasaki H., Tanaka K., Okuda K., 2006a. Professional oral care reduces influenza infection in elderly. Archives of Gerontology and Geriatrics 43: 157-164.
  2. Abe S., Ishihara K., Adachi M., Okuda K., 2006b. Oral hygiene evaluation for effective oral care in preventing pneumonia in dentate elderly. Archives of Gerontology and Geriatrics 43: 53-64.
  3. Abe S., Ishihara K., Adachi M., Okuda K., 2008. Tonguecoating as risk indicator for aspiration pneumonia in edentate elderly. Archives of Gerontology and Geriatrics 47 (2): 267-275.
  4. Huang Y., Holzel R., Pethig R., Wang X-B., 1992. Differences in the AC electrodynamics of viable and non-viable yeast cells determined through combined dielectrophoresis and electrorotation studies. Phys. Med. Biol. 37 (7): 1499-1517.
  5. Jones T. B., 1995. Electromechanics of Particles, Cambridge University Press. New York, 1st edition.
  6. Llamas M., Giner V., Sancho M., 1998. The dynamic evolution of cell chaining in a biological suspension induced by an electrical field. J. Phys. D: Appl. Phys. 31 (21): 3160-3167.
  7. Neyraud E., Bult J. H. F., Dransfield E., 2009. Continuous analysis of parotid saliva during resting and shortduration simulated chewing. Archives of oral biology 54 (5): 449-456.
  8. Suehiro J., Yatsunami R., Hamada R., Hara M., 1999. Quantitative estimation of biological cell concentration suspended in aqueous medium by using dielectrophoretic impedance measurement method. J. Phys. D: Appl. Phys. 32: 2814-2820.
  9. Wang X-B., Huang Y., Burt J. P. H., Markx G. H., Pethig R., 1993. Selective dielectrophoretic confinement of bioparticles in potential energy wells. J. Phys. D: Appl. Phys. 26 (8): 1278-1285.
Download


Paper Citation


in Harvard Style

Hamada R. and Suehiro J. (2011). OPTIMIZATION OF ELECTRIC FIELD FREQUENCY ON DIELECTROPHORETIC IMPEDANCE MEASUREMENT METHOD FOR ORAL BACTERIA DETECTION . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011) ISBN 978-989-8425-37-9, pages 125-129. DOI: 10.5220/0003128901250129


in Bibtex Style

@conference{biodevices11,
author={Ryo Hamada and Junya Suehiro},
title={OPTIMIZATION OF ELECTRIC FIELD FREQUENCY ON DIELECTROPHORETIC IMPEDANCE MEASUREMENT METHOD FOR ORAL BACTERIA DETECTION},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011)},
year={2011},
pages={125-129},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003128901250129},
isbn={978-989-8425-37-9},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011)
TI - OPTIMIZATION OF ELECTRIC FIELD FREQUENCY ON DIELECTROPHORETIC IMPEDANCE MEASUREMENT METHOD FOR ORAL BACTERIA DETECTION
SN - 978-989-8425-37-9
AU - Hamada R.
AU - Suehiro J.
PY - 2011
SP - 125
EP - 129
DO - 10.5220/0003128901250129