AUTONOMOUS SENTINELS FOR THE DETECTION OF INVASIVE PATHOGENS

Howard Clyde Wikle III, Suiqiong Li, Aleksandr Simonian, Bryan A. Chin

2012

Abstract

This paper describes the results of a research project to investigate and develop an autonomous pathogen detection and capture system that mimics the function of naturally occurring biological defensive systems, such as white blood cells. The autonomous sentinel system is envisioned to have the capability of seeking out invasive pathogens in liquid environments, detecting and capturing them. Once detected and captured the invasive pathogens can be removed, by retrieving the sentinels using a magnetic field. The sentinels are composed of two main parts: a magnetoelastic resonator whose motion and detection functions is actuated and monitored using magnetic fields; and a bio-probe that is immobilized onto the resonator surface and captures specific target pathogens. The freestanding sentinels require no on-board power for motion or to signal detection of a target pathogen. Upon contact with the target pathogen, the bio-molecular recognition element on the sentinel will bind with the target cell. This will cause a mass change of the sentinel, which results in a change in the sentinel's resonant frequency and the instantaneous detection of the target pathogen. Similar to white blood cells, the autonomous sentinels when placed in a liquid analyte will move through the analyte, capture and disable the target pathogens and signal their detection. The objective of this paper is to demonstrate proof-in-principal of the concept of autonomous sentinels.

References

  1. Ballantine, D. S. Jr, White, R. M., Martin, S. J., Ricco, A. J., Zellers, T., Frye, G. C., Wohltjen, H., 1997. Acoustic wave sensors: Theory, design, & physiochemical applications, Academic Press. San Diego, CA.
  2. Brigati, J. R., Petrenko, V. A., 2005. Thermostability of landscape phage probes. Analytical and Bioanalytical Chemistry, 382, pp.1346-50.
  3. Grimes, C. A., Stoyanov, P. G., Kouzoudis, D., Ong, K. G., 1999. Remote query pressure measurement using magnetoelastic sensors. Review of Scientific Instruments, 70, pp.4711-14.
  4. Landau, L. D., Lifshitz, E. M., 1986. Theory of elasticity, Pergamon Press. Oxford, 3rd edition.
  5. Liang, C., Morshed, S., Prorok, B. C., 2007. Correction for longitudinal mode vibration in thin slender beams. Applied Physics Letters, 90, pp.221912.
  6. Olofsson, L., Ankarloo, J., Andersson, P. O., Nicholls, I. A., 2001. Filamentous bacteriophage stability in nonaqueous media. Chemistry & Biology, 8, pp.661-71.
  7. Petrenko, V. A., 2008. Evolution of phage display: from bioactive peptides to bioselective nanomaterials. Expert Opinion on Drug Delivery, 5, pp.825-36.
  8. Petrenko, V. A., Smith, G. P., 2000. Phages from landscape libraries as substitute antibodies. Protein Engineering, 13, pp.589-92.
  9. Petrenko, V. A., Sorokulova, I. B., 2004. Detection of biological threats. A challenge for directed molecular evolution. Journal of Microbiological Methods, 58, pp.147-68.
  10. Sorokulova, I. B., Olsen, E. V., Chen, I. H., Fiebor, B., Barbaree, J. M., Vodyanoy, V. J., Chin, B. A., Petrenko, V. A., 2005. Landscape phage probes for Salmonella typhimurium. Journal of Microbiological Methods, 63, pp.55-72.
  11. Wetzel, B., Schaefer, H., 1982. National Cancer Institute.
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Paper Citation


in Harvard Style

Wikle III H., Li S., Simonian A. and A. Chin B. (2012). AUTONOMOUS SENTINELS FOR THE DETECTION OF INVASIVE PATHOGENS . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2012) ISBN 978-989-8425-91-1, pages 47-52. DOI: 10.5220/0003775200470052


in Bibtex Style

@conference{biodevices12,
author={Howard Clyde Wikle III and Suiqiong Li and Aleksandr Simonian and Bryan A. Chin},
title={AUTONOMOUS SENTINELS FOR THE DETECTION OF INVASIVE PATHOGENS},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2012)},
year={2012},
pages={47-52},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003775200470052},
isbn={978-989-8425-91-1},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2012)
TI - AUTONOMOUS SENTINELS FOR THE DETECTION OF INVASIVE PATHOGENS
SN - 978-989-8425-91-1
AU - Wikle III H.
AU - Li S.
AU - Simonian A.
AU - A. Chin B.
PY - 2012
SP - 47
EP - 52
DO - 10.5220/0003775200470052