Instrumental Tools for Express Analysis of Lacrimal Fluids

Valentina Vassilenko, Mónica Silva, Ricardo Alves, João O’Neill

2013

Abstract

Analysis of lacrimal fluid is under attention of scientists and physicians as an open “window” for non-invasive assessment to relevant information about the health status. In the present work we propose an innovative method for the analysis of volatile metabolites present in the lacrimal fluid by non-invasive, fast and inexpensive technique: Ion Mobility Spectrometry coupled to a Multi-Capillary Column (MCC-IMS). Experimental protocol for lacrimal fluid collection and its further analysis by MCC-IMS was developed. For the first time this technology was used for the analysis of tears from healthy and diabetic person for a “proof of concept” purpose. Obtained experimental result showed that proposed method is suitable for the sensitive in-situ express analysis of Volatile Organic Compounds (VOCs) from lacrimal fluid and have a promising diagnostic potential.

References

  1. Baumbach, J. (2009). Ion mobility spectrometry coupled with multi-capillary columns for metabolic profiling of human breath. In Journal of Breath Research. IOP Publishing.
  2. Bensch, H. and Leonhardt, M. (2002) Comparison of drift times of different IMS. Ion Mobility Spectrometry., 5 (3), 7-10
  3. Blake, R. S., Monks, P. S., & Ellis, A. M. (June de 2009). Proton-Transfer Reaction. ChemInform.
  4. Eiceman, G. (2005). Ion Mobility Spectrometry. Taylor & Francis Group.
  5. Spanel, P., & Smith, D. (November de 1996). Selected ion flow tube: a technique for quantitative trace gas. Medical & biological engineering & computing, pp. 409-419.
  6. Stach, J., & Baumbach, J. I. (2002). Ion Mobility Spetrometry - Basic Elements and Applications. International Journal of Ion Mobility Spectrometry, pp. 1-21.
  7. Stauffer, E., Dolan, J. A., & Newman, R. (2007). Fire Debris Analysis. Academic Press.
  8. Zhou, L., and Beuerman, R. W. (2012). Tear analysis in ocular surface diseases. Progress in Retinal and Eye Research, pp. 1-24.
Download


Paper Citation


in Harvard Style

Vassilenko V., Silva M., Alves R. and O’Neill J. (2013). Instrumental Tools for Express Analysis of Lacrimal Fluids . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2013) ISBN 978-989-8565-34-1, pages 220-224. DOI: 10.5220/0004329602200224


in Bibtex Style

@conference{biodevices13,
author={Valentina Vassilenko and Mónica Silva and Ricardo Alves and João O’Neill},
title={Instrumental Tools for Express Analysis of Lacrimal Fluids},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2013)},
year={2013},
pages={220-224},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0004329602200224},
isbn={978-989-8565-34-1},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2013)
TI - Instrumental Tools for Express Analysis of Lacrimal Fluids
SN - 978-989-8565-34-1
AU - Vassilenko V.
AU - Silva M.
AU - Alves R.
AU - O’Neill J.
PY - 2013
SP - 220
EP - 224
DO - 10.5220/0004329602200224