Single Cell Array Impedance Analysis for Cell Detection and Classification in a Microfluidic Device

Emre Altinagac, Selen Taskin, Hüseyin Kizil

2017

Abstract

Impedance analysis of single cells is presented in this paper. Following the separation of a target cell type by dielectrophoresis in our previous work, this paper focuses on capturing the cells as a single array and performing impedance analysis to point out the signature difference between each cell type. Lab-on-a-chip devices having a titanium interdigitated electrode layer on a glass substrate and a PDMS microchannel are fabricated to capture each cell in a single form and perform impedance analysis. MDA-MB-231 and HeLa cells are used in our experiments.

References

1. Mateo, J., Gerlinger, M., Rodrigues, D., de Bono, J. S., 2014. The promise of circulating tumor cell analysis in cancer management. Genome Biol, 15(8), 448.
2. Karabacak, N. M., Spuhler, P. S., Fachin, F., Lim, E. J., Pai, V., Ozkumur, E., Toner, M., 2014. Microfluidic, marker-free isolation of circulating tumor cells from blood samples. Nature protocols, 9(3), 694-710.
3. Alix-Panabières, C., Pantel, K., 2014. Technologies for detection of circulating tumor cells: facts and vision. Lab on a Chip, 14(1), 57-62
4. Jin, C., McFaul, S. M., Duffy, S. P., Deng, X., Tavassoli, P., Black, P. C., Ma, H., 2014. Technologies for labelfree separation of circulating tumor cells: from historical foundations to recent developments. Lab on a Chip, 14(1), 32-44.
5. Hajba, L., Guttman, A., 2014. Circulating tumor-cell detection and capture using microfluidic devices. TrAC Trends in Analytical Chemistry, 59, 9-16.
6. Park, H., Kim, D., Yun, K.S., 2010. Single-cell manipulation on microfluidic chip by dielectrophoretic actuation and impedance detection. Sensors and Actuators B: Chemical, 150(1), 167-173.
7. Anh-Nguyen, T., Tiberius, B., Pliquett, U., & Urban, G. A., 2016. An impedance biosensor for monitoring cancer cell attachment, spreading and drug-induced apoptosis. Sensors and Actuators A: Physical, 241, 231-237.
8. Dastider, S. G., Barizuddin, S., Yuksek, N., Dweik, M., & Almasri, M., 2016. Impedance biosensor for rapid detection of low concentration of E. coli 0157: H7. In 2016 IEEE 29th International Conference on Micro Electro Mechanical Systems (MEMS) (pp. 302-306). IEEE.
9. Tan, W.H., Takeuchi, S., 2007. A trap-and-release integrated microfluidic system for dynamic microarray applications. Proceedings of the National Academy of Sciences, 104(4), 1146-1151.
10. Zhou, Y., Basu, S., Laue, E., & Seshia, A. A., 2016. Single cell studies of mouse embryonic stem cell (mESC) differentiation by electrical impedance measurements in a microfluidic device. Biosensors and Bioelectronics, 81, 249-258.

Paper Citation

in Harvard Style

Altinagac E., Taskin S. and Kizil H. (2017). Single Cell Array Impedance Analysis for Cell Detection and Classification in a Microfluidic Device . In Proceedings of the 10th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2017) ISBN 978-989-758-216-5, pages 49-53. DOI: 10.5220/0006166700490053

in Bibtex Style

@conference{biodevices17,
author={Emre Altinagac and Selen Taskin and Hüseyin Kizil},
title={Single Cell Array Impedance Analysis for Cell Detection and Classification in a Microfluidic Device},
booktitle={Proceedings of the 10th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2017)},
year={2017},
pages={49-53},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0006166700490053},
isbn={978-989-758-216-5},
}

in EndNote Style

TY - CONF
JO - Proceedings of the 10th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2017)
TI - Single Cell Array Impedance Analysis for Cell Detection and Classification in a Microfluidic Device
SN - 978-989-758-216-5
AU - Altinagac E.
AU - Taskin S.
AU - Kizil H.
PY - 2017
SP - 49
EP - 53
DO - 10.5220/0006166700490053