Comparison of Performance between Mannitol Salt Agar-supplemented Cefoxitin Disc and Chromogenic Media for Methicillin-resistance Staphylococcus Aureus Screening

Sri Amelia, Dian Dwi Wahyuni, Rina Yunita, Muhammad Fakhrur Rozi

2018

Abstract

Methicillin-resistance Staphylococcus aureus (MRSA) and its burden still become a major problem in hospital setting worldwide. Accurate and rapid screening method is needed to prevent transmission We conducted a study to compare the performance between conventional method, modified-conventional method, and chromogenic media to detect MRSA carrier. This cross-sectional study was obtained 80 nasal swabs of medical personnel who worked in Intensive Care Unit. The location of study was in Hasan Sadikin General Hospital and Sentosa Hospital, Bandung, Indonesia between March and July 2009. Meanwhile, incubation and identification process were set in Department of Microbiology, Universitas Padjajaran, Bandung, Indonesia. Modified-conventional method, cefoxitin disc plated at the bottom of Mannitol-Salt media, was made. The result showed that specificity increased (100%, p-value < 0.001, kappa index= 1.00) using modified media and similar result was also found with chromogenic media. While using conventional method alone just produced 98.7% of specificity and 100% of sensitivity (p-value <0.001, kappa index=0.85). Thus, modified-conventional method can be considerable since its detection rate of MRSA similarly found as a reliable method for MRSA screening.

Paper Citation

in Harvard Style

Amelia S., Wahyuni D., Yunita R. and Rozi M. (2018). Comparison of Performance between Mannitol Salt Agar-supplemented Cefoxitin Disc and Chromogenic Media for Methicillin-resistance Staphylococcus Aureus Screening.In Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR, ISBN 978-989-758-449-7, pages 476-480. DOI: 10.5220/0010075004760480

in Bibtex Style

@conference{icosteerr18,
author={Sri Amelia and Dian Dwi Wahyuni and Rina Yunita and Muhammad Fakhrur Rozi},
title={Comparison of Performance between Mannitol Salt Agar-supplemented Cefoxitin Disc and Chromogenic Media for Methicillin-resistance Staphylococcus Aureus Screening},
booktitle={Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR,},
year={2018},
pages={476-480},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010075004760480},
isbn={978-989-758-449-7},
}

in EndNote Style

TY - CONF

JO - Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR,
TI - Comparison of Performance between Mannitol Salt Agar-supplemented Cefoxitin Disc and Chromogenic Media for Methicillin-resistance Staphylococcus Aureus Screening
SN - 978-989-758-449-7
AU - Amelia S.
AU - Wahyuni D.
AU - Yunita R.
AU - Rozi M.
PY - 2018
SP - 476
EP - 480
DO - 10.5220/0010075004760480