Interleukin-4 Gene Polymorphisms in Type 2 Diabetes Mellitus in Medan, Indonesia

Milahayati Daulay, Mutiara Indah Sari

2018

Abstract

Chronic hyperglycemia in type 2 diabetes mellitus is associated with the production of proinflammatory cytokines such as Interleukin (IL)-1, Tumor Necrosis Factor (TNF), Interferon (IFN)-c, IL-6, IL-12, and IL- 18. This inflammatory condition triggers the formation of anti-inflammatory cytokines such as IL-4, IL-10, IL-13, and IFN-α that work antagonistically against the pro-inflammatory cytokines. Interleukin-4 gene polymorphisms are associated with inflammatory events arising from hyperglycemic conditions. This study aims to determine the polymorphism of IL-4 -33 C>T gene in diabetic patients. A total of 40 diabetic patients from Universitas Sumatera Utara Hospital, Medan were genotyped for IL-4 SNPs (single nucleotide polymorphisms) -33 C>T using restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR). Genotype frequencies were calculated in patients by direct gene counting. The most frequent genotype in diabetic patients from Universitas Sumatera Utara Hospital in Medan was TT in 20 patients (50%). The frequency of CT genotype was 18 (45%) and CC genotype was 2 (5%). Previous studies have shown differences in genotype frequencies in other regions indicating a genetic factor difference in diabetes mellitus. Based on the result of present study it may be concluded that the functional gene polymorphisms of IL-4 play an important role in type 2 diabetes mellitus.

Paper Citation

in Harvard Style

Daulay M. and Sari M. (2018). Interleukin-4 Gene Polymorphisms in Type 2 Diabetes Mellitus in Medan, Indonesia.In Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR, ISBN 978-989-758-449-7, pages 575-577. DOI: 10.5220/0010078905750577

in Bibtex Style

@conference{icosteerr18,
author={Milahayati Daulay and Mutiara Indah Sari},
title={Interleukin-4 Gene Polymorphisms in Type 2 Diabetes Mellitus in Medan, Indonesia},
booktitle={Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR,},
year={2018},
pages={575-577},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010078905750577},
isbn={978-989-758-449-7},
}

in EndNote Style

TY - CONF

JO - Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR,
TI - Interleukin-4 Gene Polymorphisms in Type 2 Diabetes Mellitus in Medan, Indonesia
SN - 978-989-758-449-7
AU - Daulay M.
AU - Sari M.
PY - 2018
SP - 575
EP - 577
DO - 10.5220/0010078905750577