Role Selective and Nonselective Media for Isolation of Burkholderia Species from Patients with Suspected Melioidosis

R. Lia Kusumawati, Mirzan Hasibuan, Afrinayanti W. Siregar, Tryna Tania

2018

Abstract

Melioidosis is an infectious disease caused by the Burkholderia bacteria species, especially Burkholderia pseudomallei and Burkholderia cepacia, disease is endemic in Southeast Asia and Northern Australia. As a tropical country like Indonesia, this is a very serious global threat. This study aims to compare selective and non-selective media in diagnosing Melioidosis based on the results of culture of clinical specimens of patients with suspected Melioidosis. The results showed that as many as 112 (100%) of suspected clinical samples of bacterial melioidosis were grown on nonselective media, Mac-Conkey agar and 110 (98.2%) bacteria growing on Columbia agar agar medium. While on Ashdown’s Selective Agar (ASA) there is bacterial growth of 10 samples (8.9%.). Where 7 of 10 samples are is Burkholderia species (Burkhoderia cepacia 5 and Burkholderia pseudomallei 2). The results of this study indicate that Ashdown's media plays an important role in selecting Burkholderia species as the cause of Melioidosis.

Paper Citation

in Harvard Style

Kusumawati R., Hasibuan M., Siregar A. and Tania T. (2018). Role Selective and Nonselective Media for Isolation of Burkholderia Species from Patients with Suspected Melioidosis.In Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR, ISBN 978-989-758-449-7, pages 582-585. DOI: 10.5220/0010079305820585

in Bibtex Style

@conference{icosteerr18,
author={R. Lia Kusumawati and Mirzan Hasibuan and Afrinayanti W. Siregar and Tryna Tania},
title={Role Selective and Nonselective Media for Isolation of Burkholderia Species from Patients with Suspected Melioidosis},
booktitle={Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR,},
year={2018},
pages={582-585},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010079305820585},
isbn={978-989-758-449-7},
}

in EndNote Style

TY - CONF

JO - Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR,
TI - Role Selective and Nonselective Media for Isolation of Burkholderia Species from Patients with Suspected Melioidosis
SN - 978-989-758-449-7
AU - Kusumawati R.
AU - Hasibuan M.
AU - Siregar A.
AU - Tania T.
PY - 2018
SP - 582
EP - 585
DO - 10.5220/0010079305820585