Phenotypic and Molecular Detection of OXA-48 Gene Carbapenem-resistant Klebsiella Pneumoniae and Escherichia Coli Isolates in Haji Adam Malik Hospital Medan, Indonesia

Mirzan Hasibuan, R. Lia Kusumawati, Dwi Suryanto

2018

Abstract

Carbapenem-resistant to Klebsiella pneumoniae and Escherichia coli, increasingly reported as a major cause of infection in hospitals and healthcare facilities. Carbapenemase is an enzyme produced by gram-negative bacteria that causes failure of antibiotic therapy, especially the carbapenem. The study aimed to characterize phenotypically using Vitek 2 Compact and detect OXA-48 clinical isolates of Klebsiella pneumoniae and Escherichia coli belonging to Carbapenem-Resistant using PCR. The results showed that all were betalactamase producers, which of 12 (14.11%) were resistant to carbapenem. The phenotype distribution of carbapenem is 10/12 (11.75%) Klebsiella pneumoniae and 2/12 (2.35%) Escherichia coli. From 12 isolates Carbapenemase phenotypically, in which 10 (11.77%) of both bacteria bearing OXA-48 gene with the distribution 9 (10.6%) Klebsiella pneumoniae and 1 (1.17%) Escherichia coli, respectively. The study shows that prevalence of OXA-48 genes in North Sumatra, Indonesia in Klebsiella pneumoniae and Escherichia coli which cause failure of therapeutic types of antibiotics Carbapenem. The only antibiotic that is still sensitive to carbapenem-resistant bacteria based on antimicrobial susceptibility is amikacin, which can be recommended as carbapenemases therapy.

Paper Citation

in Harvard Style

Hasibuan M., Kusumawati R. and Suryanto D. (2018). Phenotypic and Molecular Detection of OXA-48 Gene Carbapenem-resistant Klebsiella Pneumoniae and Escherichia Coli Isolates in Haji Adam Malik Hospital Medan, Indonesia.In Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR, ISBN 978-989-758-449-7, pages 590-594. DOI: 10.5220/0010079805900594

in Bibtex Style

@conference{icosteerr18,
author={Mirzan Hasibuan and R. Lia Kusumawati and Dwi Suryanto},
title={Phenotypic and Molecular Detection of OXA-48 Gene Carbapenem-resistant Klebsiella Pneumoniae and Escherichia Coli Isolates in Haji Adam Malik Hospital Medan, Indonesia},
booktitle={Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR,},
year={2018},
pages={590-594},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010079805900594},
isbn={978-989-758-449-7},
}

in EndNote Style

TY - CONF

JO - Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR,
TI - Phenotypic and Molecular Detection of OXA-48 Gene Carbapenem-resistant Klebsiella Pneumoniae and Escherichia Coli Isolates in Haji Adam Malik Hospital Medan, Indonesia
SN - 978-989-758-449-7
AU - Hasibuan M.
AU - Kusumawati R.
AU - Suryanto D.
PY - 2018
SP - 590
EP - 594
DO - 10.5220/0010079805900594