Modeling of C-SNARF-1 pH Fluorescence Properties: Towards Calibration Free Optical Fiber pH Sensing for in Vivo Applications

Rutjaphan Kateklum, Bernard Gauthier-Manuel, Christian Pieralli, Samlee Mankhetkorn, Bruno Wacogne, Bruno Wacogne

2018

Abstract

Organic functions of the human body are related to biological constants. Variations of these constants, among them pH, induce pathological troubles. The general goal of our work is to fabricate a fluorescent pH sensor at the end of an optical fiber for in vivo pH measurements. One difficulty using fluorescence indicators is the need to perform an accurate calibration. In this communication, we present methods used to simplify and potentially avoid calibration procedures of fluorescence indicators. The first method concerns the simplification of calibration procedures making them independent of the indicator’s concentration, path length and equipment used. The second method concerns modelling the fluorescence emission of the molecules as a function of pH only. This model is used to fit the exact shape of C-SNARF-1 fluorescence spectra obtained at any pH. Subsequently, the pH of a solution can be computed with an accuracy of 0.1 pH unit without the calibration procedure employed up to now. These methods constitute the first steps toward calibration free pH measurements. They can be applied to any fluorescent indicator exhibiting a dual emission peak. As a conclusion, this is the first time that fluorescence properties of C-SNARF-1 are fully mathematically described.

Paper Citation

in Harvard Style

Kateklum R., Gauthier-Manuel B., Pieralli C., Mankhetkorn S. and Wacogne B. (2018). Modeling of C-SNARF-1 pH Fluorescence Properties: Towards Calibration Free Optical Fiber pH Sensing for in Vivo Applications. In Proceedings of the 11th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2018) - Volume 1: BIODEVICES; ISBN 978-989-758-277-6, SciTePress, pages 17-24. DOI: 10.5220/0006514300170024

in Bibtex Style

@conference{biodevices18,
author={Rutjaphan Kateklum and Bernard Gauthier-Manuel and Christian Pieralli and Samlee Mankhetkorn and Bruno Wacogne},
title={Modeling of C-SNARF-1 pH Fluorescence Properties: Towards Calibration Free Optical Fiber pH Sensing for in Vivo Applications},
booktitle={Proceedings of the 11th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2018) - Volume 1: BIODEVICES},
year={2018},
pages={17-24},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0006514300170024},
isbn={978-989-758-277-6},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 11th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2018) - Volume 1: BIODEVICES
TI - Modeling of C-SNARF-1 pH Fluorescence Properties: Towards Calibration Free Optical Fiber pH Sensing for in Vivo Applications
SN - 978-989-758-277-6
AU - Kateklum R.
AU - Gauthier-Manuel B.
AU - Pieralli C.
AU - Mankhetkorn S.
AU - Wacogne B.
PY - 2018
SP - 17
EP - 24
DO - 10.5220/0006514300170024
PB - SciTePress