Differences Expression of Caspase-3 In Hepar and Spleen of Rattus norvegicus Infected with Methicillin Resistant Staphylococcus aureus and Enterococcus faecalis

Sri Wahyuni, Yoes Prijatna Dachlan, Agung Dwi Wahyu Widodo

2018

Abstract

Bacteria are organisms that can cause infection. Methicillin Resistant Staphylococcus aureus (MRSA) and Entrerococcus faecalis are gram-positive bacteria that can cause nosocomial infections. Virulence factors of MRSA thus play a role in the infection process, such as are polysaccharide, surface proteins such as adhesins, glycoprotein, hemagglutinin, and fibronectin, while enterococcus such as gelatinase, cytolysin, enterococcal surface protein (Esp) and aggregation substance (AS) are the virulence factors of Enterococcus that can cause infection. This research is True Experimental research with post-test only for the Control Group Design. The MRSA and E. faecalis bacteria were injected intraperitoneally to R. norvegicus and observed after 24 hours. Animals were placed into three groups: the control group, treatment with MRSA, and Enterococcus faecalis. The hepar and spleen were isolated from the dead R. norvegicus and a Immunohistochemistry (IHC) test was conducted to observe the expression of caspase-3 by light microscope. The result showed that the caspase-3 expression increased in the infected group of MRSA and Enterococcus faecalis compared with the control group. The expression of caspase-3 in the hepar was higher than in the spleen. The hepar serves as the receiver of the portal and systemic circulation. The hepar also plays an important role in the host defense that is exposed to and will catch pathogens, followed by cleaning.  The increased expression of caspase-3 suggests the cell death also increased.

Paper Citation

in Harvard Style

Wahyuni S., Dachlan Y. and Wahyu Widodo A. (2018). Differences Expression of Caspase-3 In Hepar and Spleen of Rattus norvegicus Infected with Methicillin Resistant Staphylococcus aureus and Enterococcus faecalis.In Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS, ISBN 978-989-758-348-3, pages 253-257. DOI: 10.5220/0007540902530257

in Bibtex Style

@conference{icps18,
author={Sri Wahyuni and Yoes Prijatna Dachlan and Agung Dwi Wahyu Widodo},
title={Differences Expression of Caspase-3 In Hepar and Spleen of Rattus norvegicus Infected with Methicillin Resistant Staphylococcus aureus and Enterococcus faecalis},
booktitle={Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,},
year={2018},
pages={253-257},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007540902530257},
isbn={978-989-758-348-3},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,
TI - Differences Expression of Caspase-3 In Hepar and Spleen of Rattus norvegicus Infected with Methicillin Resistant Staphylococcus aureus and Enterococcus faecalis
SN - 978-989-758-348-3
AU - Wahyuni S.
AU - Dachlan Y.
AU - Wahyu Widodo A.
PY - 2018
SP - 253
EP - 257
DO - 10.5220/0007540902530257