Resistance Test of Vibrio Sp from Milkish (Chanos chanos) in Fishpond Jabon Sidoarjo to Heavy Metals and Antibiotics

Pratikah Verdianti, Fitriyati Mukhlishoh, Tuty Putri, Ayu Puspitasari

2018

Abstract

The milkfish (Chanos chanos) obtained from Fishponds Jabon Sidoarjo contained heavy metals such as Pb and Cd. Heavy metals pollution can be controlled by bioremediation. The objective of this research is to determine the resistance of Vibrio sp isolated from milkfish to heavy metals Pb, Cd, at various concentrations and to know the resistance of these bacteria to antibiotics such as tetracycline and chloramphenicol. Resistance tests of Vibrio sp were performed using dilution and pour plate method. The Nutrient agar media used in this study contained heavy metals PbCl2 and CdCl2 with concentrations of 5 ppm, 10 ppm, 25 ppm. Heavy metals-resistant Vibrio sp was tested its antibiotic resistance with diffusion method using antibiotic disks such as Tetracycline 30 μg and Chloramphenicol 30 μg. The results showed that Vibrio sp was resistant to heavy metals Pb and Cd at all concentrations. Antibiotic sensitivity test using chloramphenicol 30 μg showed that Vibrio sp was resistant to chloramphenicol at concentration of 5ppm. Pb-resistant Vibrio sp was shown resistant to chloramphenicol at concentration of 10 ppm and 25 ppm. Pb- and Cd-resistant Vibrio sp were shown resistant to Tetracycline 30 μg at all concentrations.

Paper Citation

in Harvard Style

Verdianti P., Mukhlishoh F., Putri T. and Puspitasari A. (2018). Resistance Test of Vibrio Sp from Milkish (Chanos chanos) in Fishpond Jabon Sidoarjo to Heavy Metals and Antibiotics.In Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS, ISBN 978-989-758-348-3, pages 371-375. DOI: 10.5220/0007543203710375

in Bibtex Style

@conference{icps18,
author={Pratikah Verdianti and Fitriyati Mukhlishoh and Tuty Putri and Ayu Puspitasari},
title={Resistance Test of Vibrio Sp from Milkish (Chanos chanos) in Fishpond Jabon Sidoarjo to Heavy Metals and Antibiotics},
booktitle={Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,},
year={2018},
pages={371-375},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007543203710375},
isbn={978-989-758-348-3},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,
TI - Resistance Test of Vibrio Sp from Milkish (Chanos chanos) in Fishpond Jabon Sidoarjo to Heavy Metals and Antibiotics
SN - 978-989-758-348-3
AU - Verdianti P.
AU - Mukhlishoh F.
AU - Putri T.
AU - Puspitasari A.
PY - 2018
SP - 371
EP - 375
DO - 10.5220/0007543203710375