Profile of IgM Anti PGL-1 and IgM Anti LID1-NDO on Leprosy Patients of Dr. Soetomo General Hospital, Surabaya

Iswahyudi, D. Adriaty, Prita P. K., I. Agusni, I. Agusni, S. Izumi, C. R. S. Prakoeswa, C. R. S. Prakoeswa

2018

Abstract

Serological tests have been used to assist leprosy disease management. The latest serological test towards anti LID1-NDO ((Leprosy IDRI Diagnostic 1- Natural Disaccharide Octyl) challenges the leprosy antigen already in use, PGL-1, to provide a better, more sensitive leprosy serological test. This research is to study the profile of IgM anti PGL-1 compared to IgM anti LID1-NDO in leprosy patients from Surabaya. Sera sample 91 leprosy patients from Dr. Soetomo Hospital, Surabaya (PB : 30, MB: 61) and tested against IgM anti PGL-1 and IgM anti LID1-NDO using the ELISA technique. The result of anti PGL-1 is stated in u/mL and in optical density (OD) unit for IgM anti LID1-NDO. The average IgM anti PGL-1 titer in PB patients are 1577.1 u/ml, whereas for anti LID1-NDO antibody the average OD is 0.166. In MB patients IgM anti PGL-1 has the average of 3629.2 u/ml. The absorbance of IgM anti LID1-NDO was 0.228 on average. The majority of MB patients presented with positive anti LID1-NDO and anti PGL-I responses (77.05 % and 68.85 %). For PB patients presented with positive anti LID1-NDO and anti PGL-I responses (70% and 60%). strong correlation was found (P=0.003) between IgM anti LID1-NDO and anti PGL-1. PGL-1 originates from the polysaccharide of M. leprae, and LID1-NDO fuses this polysaccharide with another set of protein LID1-NDO is potential to be used in serological tests for leprosy diagnosis and monitoring, as it performs to a similar manner to PGL-1.

Paper Citation

in Harvard Style

Iswahyudi., Adriaty D., K. P., Agusni I., Izumi S. and Prakoeswa C. (2018). Profile of IgM Anti PGL-1 and IgM Anti LID1-NDO on Leprosy Patients of Dr. Soetomo General Hospital, Surabaya.In Proceedings of the 23rd Regional Conference of Dermatology - Volume 1: RCD, ISBN 978-989-758-494-7, pages 320-322. DOI: 10.5220/0008156603200322

in Bibtex Style

@conference{rcd18,
author={Iswahyudi and D. Adriaty and Prita P. K. and I. Agusni and S. Izumi and C. R. S. Prakoeswa},
title={Profile of IgM Anti PGL-1 and IgM Anti LID1-NDO on Leprosy Patients of Dr. Soetomo General Hospital, Surabaya},
booktitle={Proceedings of the 23rd Regional Conference of Dermatology - Volume 1: RCD,},
year={2018},
pages={320-322},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008156603200322},
isbn={978-989-758-494-7},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 23rd Regional Conference of Dermatology - Volume 1: RCD,
TI - Profile of IgM Anti PGL-1 and IgM Anti LID1-NDO on Leprosy Patients of Dr. Soetomo General Hospital, Surabaya
SN - 978-989-758-494-7
AU - Iswahyudi.
AU - Adriaty D.
AU - K. P.
AU - Agusni I.
AU - Izumi S.
AU - Prakoeswa C.
PY - 2018
SP - 320
EP - 322
DO - 10.5220/0008156603200322