Antihiperlipidemic Activity of the Methanolic Extract of Parijoto (Medinilla speciosa) on the Protein Profile of Hyperlipidemic Rats

Noor Nailis Sa'adah, Awik Puji Dyah Nurhayati

2018

Abstract

High-fat diets and frequent feeding contributes to the onset of hyperlipidemia, a family of disorders that is characterised by abnormally high levels of lipids in the blood. Hyperlipidemia is a major cause of atherosclerosis, which is related to conditions such as coronary heart disease (CHD), ischemic cerebrovascular disease, peripheral vascular disease and pancreatitis. Parijoto (M. speciosa) is an endemic plant in Asia with a distribution center in Malaysia, Indonesia and Philippines that is generally consumed by pregnant women and used to treat diarrhoea and cholesterol. The parijoto fruit contains a flavonoid compound which has been suggested to decrease the risk of coronary heart disease, inflammatory process, and atherosclerosis through their antioxidant activities. During pathological conditions, there are differences in the protein profiles that indicate the presence of protein biomarkers. Different levels of protein expression serves as a biomarker of disease progression. This study aims to determine the effects of the methanolic extract of parijoto (M. speciosa) on the protein profile of hyperlipidemic rats. Rats were divided into five groups: normal rats, hyperlipidemic rats, and hyperlipidemic rats that were given the methanolic extract of parijoto (M. speciosa) at 500 mg •kg ˗1, 1000 mg • kg ˗1, and 1500 mg • kg˗1 body weight. Rats were terminated after the 30 day treatment of the methanolic extract of parijoto (M. speciosa) and their blood collected. Protein profile was determined by the Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) method. Results showed that the proteins that appeared in each group were proteins with the molecular weight 160; 144; 131; 124; 117; 110; 93; 76; 59; 52; 49; 42; 33; 25 and 14 kDa, however the(control) protein 117 kDa was not present in group I. Protein 117 kDa was presumed as the sterol regulatory element binding protein-1c (His-SREBP-1c), the transcription factor that transduces the insulin signal

Paper Citation

in Harvard Style

Sa'adah N. and Nurhayati A. (2018). Antihiperlipidemic Activity of the Methanolic Extract of Parijoto (Medinilla speciosa) on the Protein Profile of Hyperlipidemic Rats. In Proceedings of the Built Environment, Science and Technology International Conference - Volume 1: BEST ICON, ISBN 978-989-758-414-5, pages 123-128. DOI: 10.5220/0008907900002481

in Bibtex Style

@conference{best icon18,
author={Noor Nailis Sa'adah and Awik Puji Dyah Nurhayati},
title={Antihiperlipidemic Activity of the Methanolic Extract of Parijoto (Medinilla speciosa) on the Protein Profile of Hyperlipidemic Rats},
booktitle={Proceedings of the Built Environment, Science and Technology International Conference - Volume 1: BEST ICON,},
year={2018},
pages={123-128},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008907900002481},
isbn={978-989-758-414-5},
}

in EndNote Style

TY - CONF

JO - Proceedings of the Built Environment, Science and Technology International Conference - Volume 1: BEST ICON,
TI - Antihiperlipidemic Activity of the Methanolic Extract of Parijoto (Medinilla speciosa) on the Protein Profile of Hyperlipidemic Rats
SN - 978-989-758-414-5
AU - Sa'adah N.
AU - Nurhayati A.
PY - 2018
SP - 123
EP - 128
DO - 10.5220/0008907900002481