Cell Cycle Inhibition and Apoptosis Induction Activities of n-Hexane Fraction of Cyperus rotundus L. Rhizome

Masfria, Urip Harahap, Denny Satria

2018

Abstract

Background: Breast cancer is one of the leading cause of death and the most common cancer type amongst women in the world after cervic cancer. Objective: To evaluate the cell cycle inhibition and apoptosis induction activities on T47D cell lines of n-hexane fraction (nHF) of Cyperus rotundus L. rhizomes. Methods: Ethanol extract was obtained by maceration method and was fractionated with n-hexane. Cytotoxic activity was examined with MTT assay, and cell cycle inhibition, apoptosis induction and cyclin D1 expression were assessed with flow cytometry method. Results: Cytotoxic activity of nHF was found to have IC 50 of 71.69 ± 0.34 µg/mL, nHF at concentration 35 µg/mL caused accumulation in G 0 -G 1 and S phase accumulation (56.89% and 19.36%), increased early apoptosis (26.30%) and decreased expression of cyclin D1 (26.30%). Conclusions: The results reveal that nHF of Cyperus rotundus L. rhizomes has cell cycle inhibition and apoptosis induction activities. Our further study is to isolation compounds which responsible for these activities.

Paper Citation

in Harvard Style

Masfria., Harahap U. and Satria D. (2018). Cell Cycle Inhibition and Apoptosis Induction Activities of n-Hexane Fraction of Cyperus rotundus L. Rhizome. In Proceedings of BROMO Conference - Volume 1: BROMO, ISBN 978-989-758-347-6, pages 1-1. DOI: 10.5220/0009846200002406

in Bibtex Style

@conference{bromo18,
author={Masfria and Urip Harahap and Denny Satria},
title={Cell Cycle Inhibition and Apoptosis Induction Activities of n-Hexane Fraction of Cyperus rotundus L. Rhizome},
booktitle={Proceedings of BROMO Conference - Volume 1: BROMO,},
year={2018},
pages={1-1},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0009846200002406},
isbn={978-989-758-347-6},
}

in EndNote Style

TY - CONF

JO - Proceedings of BROMO Conference - Volume 1: BROMO,
TI - Cell Cycle Inhibition and Apoptosis Induction Activities of n-Hexane Fraction of Cyperus rotundus L. Rhizome
SN - 978-989-758-347-6
AU - Masfria.
AU - Harahap U.
AU - Satria D.
PY - 2018
SP - 1
EP - 1
DO - 10.5220/0009846200002406