Genetic Diversity of Cowpea Mild Mottle Irus on Soybean in Several Region in Indonesia

Mimi Sutrawati, Sri Hendrastuti Hidayat, Bonny Purnomo Wahyu Sukarno, Gede Suastika, Ali Nurmansyah

2018

Abstract

Soybean is one of the most important food commodities in Indonesia. Virus infection on soybean has been reported worldwide as factors affecting yield loss. This study was aimed to detect Cowpea mild mottle virus (CPMMV) from several soybean cultivation areas in Java, Sumatra and Southeast Sulawesi; and further characterize their genetic variation based on nucleotide sequences of their coat protein. Several virus infection was detected using double antibody sandwhich enzyme-linked immunosorbent assay (DAS-ELISA), including CPMMV, Cucumber mosaic virus (CMV), and Soybean mosaic virus (SMV). Generally, the symptoms caused by CPMMV, CMV, and SMV are similar, Involving mottle, rugose, and vein banding. Coat protein gene of 5 CPMMV isolates (Bantul, Musi Banyuasin, Cirebon, Kendari, Cianjur) was successfully amplified and cloned. Sequence of this 5 clones of CPMMV showed high similarity, ranging from 88.2 to 99.8%; whereas their sequence homology to those of Taiwan and China ranging from 88.2 to 98.6%. Phylogenetic analysis showed different clusters of CPMMV Indonesian isolates: isolates from Bantul, Cirebon, Musi Banyuasin (Palembang) is clustered with Taiwan isolate (JX020701); isolate from Cianjur is clustered with China isolate (KX534092); isolate from Kendari is clustered with Puerto Rico (GU191840), Brazil (KC884247), and USA (KC774020) isolates.

Paper Citation

in Harvard Style

Sutrawati M., Hidayat S., Sukarno B., Suastika G. and Nurmansyah A. (2018). Genetic Diversity of Cowpea Mild Mottle Irus on Soybean in Several Region in Indonesia.In Proceedings of the 1st International Conference on Recent Innovations - Volume 1: ICRI, ISBN 978-989-758-458-9, pages 1815-1819. DOI: 10.5220/0009935718151819

in Bibtex Style

@conference{icri18,
author={Mimi Sutrawati and Sri Hendrastuti Hidayat and Bonny Purnomo Wahyu Sukarno and Gede Suastika and Ali Nurmansyah},
title={Genetic Diversity of Cowpea Mild Mottle Irus on Soybean in Several Region in Indonesia},
booktitle={Proceedings of the 1st International Conference on Recent Innovations - Volume 1: ICRI,},
year={2018},
pages={1815-1819},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0009935718151819},
isbn={978-989-758-458-9},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International Conference on Recent Innovations - Volume 1: ICRI,
TI - Genetic Diversity of Cowpea Mild Mottle Irus on Soybean in Several Region in Indonesia
SN - 978-989-758-458-9
AU - Sutrawati M.
AU - Hidayat S.
AU - Sukarno B.
AU - Suastika G.
AU - Nurmansyah A.
PY - 2018
SP - 1815
EP - 1819
DO - 10.5220/0009935718151819