Isolation and Characterization of Lactic Acid Bacteria from Goat Milk Kefir and Coconut Milk Kefir

Reno Permatasari Pasaribu, Emma Zaidar, Rumondang Bulan

2019

Abstract

Research about isolation and identification of LAB from goat milk kefir and coconut milk kefir has been completed. The making of goat milk kefir was used 3% kefir granule (HK) and 5% kefir granule (KK) from total volume of goat milk. As well as coconut milk kefir was used 3% kefir granule (HS) and 5% kefir granule (KS) from total volume of coconut milk. The kefir beverage, conducted quality analysis with result pH 4.2% – 4.6%, water content 89.5% (HK), 89.1% (KK), 79.2% (HS) and 78.9% (KS). Protein content 3.3% (HK), 3.1% (KK), 1.7% (HS) and 1.6% (KS), fat content 3.58% (HK), 15.2% (HS), 3.61% (KK), dan 15.8% (KS), ash content 0.79% (HK), 0.54% (HS), 0.81% (KK) and 0.9% (KS). Antibacterial test was conducted by observing the clear zone for 48 hours. Using Escherichia coli and Staphylococcus aureus as the test bacteria, resulted that sample HK showed the highest level of resistance compare to other samples. Total plate count of LAB showed that sample KK has the highest number of LAB counts for 1.7 x 108. Morphology characterization of those colony are round shape, convex elevation, off-white and also gram positive bacteria with six isolate rod shape cell, and two isolate coccus shape cell. The catalase and oxidase test showed that all isolate are negative catalase and negative oxidase.

Paper Citation

in Harvard Style

Permatasari Pasaribu R., Zaidar E. and Bulan R. (2019). Isolation and Characterization of Lactic Acid Bacteria from Goat Milk Kefir and Coconut Milk Kefir. In Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath, ISBN 978-989-758-556-2, pages 59-64. DOI: 10.5220/0010133300002775

in Bibtex Style

@conference{imc-scimath19,
author={Reno Permatasari Pasaribu and Emma Zaidar and Rumondang Bulan},
title={Isolation and Characterization of Lactic Acid Bacteria from Goat Milk Kefir and Coconut Milk Kefir},
booktitle={Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,},
year={2019},
pages={59-64},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010133300002775},
isbn={978-989-758-556-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,
TI - Isolation and Characterization of Lactic Acid Bacteria from Goat Milk Kefir and Coconut Milk Kefir
SN - 978-989-758-556-2
AU - Permatasari Pasaribu R.
AU - Zaidar E.
AU - Bulan R.
PY - 2019
SP - 59
EP - 64
DO - 10.5220/0010133300002775