Anti-fungal Activity Methanolic Extract of Calliandra calothyrsus against Storage Fungi Isolated from Dried-stored Spices

Kiki Nurtjahja, Albert Pasaribu

2019

Abstract

The methanolic extract of Calliandra calothyrsus (Leguminoceae) was conducted. The aim was to investigate the presence of flavonoid and the ability of the crude extract to inhibit mycelial growth and conidial germination of storage fungi. Eight fungal species were isolated from ten kinds of dried spices obtained from traditional markets in Medan North Sumatera. Fungal isolation was conducted using dilution method in dichloran 18% glycerol agar (DG18) medium. Plant methanolic extract obtained from 4 kg fresh leaves of Calliandra calothyrsus. The leaves were dried under shade to constant weight and ground using electrical grinder to a 1.2 kg fine powder and dissolved in ±15 l of methanol (MeOH) for 48 hours. and dried using rotary evaporator. For qualitative flavonoid determination, 10 g of fine powder of the leaves was dissolved 100 ml methanol and suspended for 12 hours, then acidified with 6% hydrochloric acid (HCl). The presence of flavonol was identified using Ultra Violet Visible Spectroscopy (UV-Vis). Results showed methanolic extract of Calliandra calothyrsus contain flavonoids group of flavonol. The crude extract have no antifungal activity againts storage fingi. However, all of the concentration inhibit conidial germination to all fungi tested.

Paper Citation

in Harvard Style

Nurtjahja K. and Pasaribu A. (2019). Anti-fungal Activity Methanolic Extract of Calliandra calothyrsus against Storage Fungi Isolated from Dried-stored Spices. In Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath, ISBN 978-989-758-556-2, pages 538-541. DOI: 10.5220/0010612600002775

in Bibtex Style

@conference{imc-scimath19,
author={Kiki Nurtjahja and Albert Pasaribu},
title={Anti-fungal Activity Methanolic Extract of Calliandra calothyrsus against Storage Fungi Isolated from Dried-stored Spices},
booktitle={Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,},
year={2019},
pages={538-541},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010612600002775},
isbn={978-989-758-556-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,
TI - Anti-fungal Activity Methanolic Extract of Calliandra calothyrsus against Storage Fungi Isolated from Dried-stored Spices
SN - 978-989-758-556-2
AU - Nurtjahja K.
AU - Pasaribu A.
PY - 2019
SP - 538
EP - 541
DO - 10.5220/0010612600002775