3D Microfluidic Perfusion Cell Culture System for Concentration Gradient and Air Bubble Trapping Functions

Dong Hyeok Park, Xuan Don Nguyen, Moon Jeong Kim, Karl Morten, Jeung Sang Go

2019

Abstract

This paper presents a cell culture well-plate for three dimensional perfusion cell cultures. A concentration gradient generator, a microchannel system, is embedded in the plate bottom for not only the perfusion culture but transfer of reagents with linear concentration gradient to 4 wells of the plate. The concentration uniformity of gradient generated is guaranteed by adding microchannel mixers at the end of generator. Sudden expansion reservoirs, air bubble traps, make perfusion cell cultures plate long-term culture without interruption of perfusion flow caused by injection of air bubbles in the microchannels. The performance of the developed 3D microfluidic perfusion cell culture system is examined experimentally and compared with analytical results. Then, it is applied to test the cytotoxicity of cells infected with Ewing’s sarcoma. Cell death is observed for different concentrations of H2O2. Finally, the 3D perfusion cell culture well-plate is presented with not only similar structure to conventional 3×4 well-plate but expansion of concentration range from a 4 fold of dilution in 4 wells to a 100 fold of dilution in 7 wells.

Paper Citation

in Harvard Style

Park D., Nguyen X., Kim M., Morten K. and Go J. (2019). 3D Microfluidic Perfusion Cell Culture System for Concentration Gradient and Air Bubble Trapping Functions. In Proceedings of the 12th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2019) - Volume 1: BIODEVICES; ISBN 978-989-758-353-7, SciTePress, pages 199-206. DOI: 10.5220/0007472001990206

in Bibtex Style

@conference{biodevices19,
author={Dong Hyeok Park and Xuan Don Nguyen and Moon Jeong Kim and Karl Morten and Jeung Sang Go},
title={3D Microfluidic Perfusion Cell Culture System for Concentration Gradient and Air Bubble Trapping Functions},
booktitle={Proceedings of the 12th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2019) - Volume 1: BIODEVICES},
year={2019},
pages={199-206},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007472001990206},
isbn={978-989-758-353-7},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 12th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2019) - Volume 1: BIODEVICES
TI - 3D Microfluidic Perfusion Cell Culture System for Concentration Gradient and Air Bubble Trapping Functions
SN - 978-989-758-353-7
AU - Park D.
AU - Nguyen X.
AU - Kim M.
AU - Morten K.
AU - Go J.
PY - 2019
SP - 199
EP - 206
DO - 10.5220/0007472001990206
PB - SciTePress