microCT for Systematic Mouse Phenotyping

Frantisek Spoutil, Michaela Prochazkova, Tereza Michalcikova, Ivana Uramova, Sarah Clewell, Vendula Novosadova, Jan Prochazka

2019

Abstract

Phenotyping of mouse mutants is one of the crucial methods for uncovering genetic network at the level of a whole organism which could help us to understand origin of rare diseases, developmental malformations, but also the process of mammalian evolution. For studying morphological aspects of either embryos or adults, the X-ray computed microtomography (microCT) has become a gold standard within the last years. The three-dimensional (3D) context, availability of data to additional analysis (e.g. volumetric, bone density, or body composition), and in-vivo approaches in the case of adults are the main advantages when compared to classic histology and bone morphology. On the other hand, the amount of data is enormous making the data storage and analysis the bottle-neck of the microCT method. To overcome this obstacle, we cooperate with bioinformatics experts to set up automation of the process at maximal possible level. Nevertheless, knowledge and experience of a specialist remain indispensable.

Paper Citation

in Harvard Style

Spoutil F., Prochazkova M., Michalcikova T., Uramova I., Clewell S., Novosadova V. and Prochazka J. (2019). microCT for Systematic Mouse Phenotyping. In Proceedings of the 12th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2019) - Volume 2: BIOIMAGING; ISBN 978-989-758-353-7, SciTePress, pages 144-149. DOI: 10.5220/0007570701440149

in Bibtex Style

@conference{bioimaging19,
author={Frantisek Spoutil and Michaela Prochazkova and Tereza Michalcikova and Ivana Uramova and Sarah Clewell and Vendula Novosadova and Jan Prochazka},
title={microCT for Systematic Mouse Phenotyping},
booktitle={Proceedings of the 12th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2019) - Volume 2: BIOIMAGING},
year={2019},
pages={144-149},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007570701440149},
isbn={978-989-758-353-7},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 12th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2019) - Volume 2: BIOIMAGING
TI - microCT for Systematic Mouse Phenotyping
SN - 978-989-758-353-7
AU - Spoutil F.
AU - Prochazkova M.
AU - Michalcikova T.
AU - Uramova I.
AU - Clewell S.
AU - Novosadova V.
AU - Prochazka J.
PY - 2019
SP - 144
EP - 149
DO - 10.5220/0007570701440149
PB - SciTePress