Therapy of Fermented Milk Lactobacillus Casei Strain Shirota to Level of Malondialdehyde (MDA) and Proteind Bands the Hearth the White Rats (Rattus Norvegicus) That given High Cholesterol Dietary

D. W. E. Putri, A. Aulanni’am, A. Srihardyastuti

2019

Abstract

Cardiovascular disease is a disease that occurs due to a disturbance in the function of the heart and blood vessels such as coronary heart disease (PJK), hypertension and stroke.One cause of PJK is a condition of hypercholesterolemia. Hypercholesterolemia is a condition which cholesterol level more than 200 mg / dL. High cholesterol dietary can cause an increase in free radicals that cause oxidative stress. Fermented milk Lactobacillus caseithought to contain biopeptida as antioxsidant.This study was to determine the effect of fermented milk Lactobacilluscasei in lowering levels of MDA and repairing protein profiles of heart. Animal that used is a rat (Rattusnorvegicus, males, aged 2-3 months, weightabout 100-250 gram.Rats divided into normal rats, hypercholesterolemia rats , hypercholesterolemia rats and therapy 1 mL and 2 mL . High cholesterol dietary used egg yolk, cholesterol pure and cholic acid that give in forcefeeding for 4 weeks.Therapy of Lactobacillus casei fermented milk given for 2 weeks in.Level of MDA was measured using the method of TBA and cardiac protein bands was tested using SDS-PAGE.The result of Research obtained showed Lactobacillus casei fermented milk therapy was significantly (p <0.05) lower levels of MDA and affect cardiac protein bands of rats. Doses of 2 mL is the best dose with decreased levels of MDA by 43.11% and is able to restore protein band profiles such as Lactobacillus caseinormal.In conclusion tha fermented milk can be used as an alternative treatment of hypercholesterolemia

Paper Citation

in Harvard Style

Putri D., Aulanni’am A. and Srihardyastuti A. (2019). Therapy of Fermented Milk Lactobacillus Casei Strain Shirota to Level of Malondialdehyde (MDA) and Proteind Bands the Hearth the White Rats (Rattus Norvegicus) That given High Cholesterol Dietary.In Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering - Volume 1: ICAMBBE, ISBN 978-989-758-483-1, pages 126-132. DOI: 10.5220/0009586901260132

in Bibtex Style

@conference{icambbe19,
author={D. W. E. Putri and A. Aulanni’am and A. Srihardyastuti},
title={Therapy of Fermented Milk Lactobacillus Casei Strain Shirota to Level of Malondialdehyde (MDA) and Proteind Bands the Hearth the White Rats (Rattus Norvegicus) That given High Cholesterol Dietary},
booktitle={Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering - Volume 1: ICAMBBE,},
year={2019},
pages={126-132},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0009586901260132},
isbn={978-989-758-483-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering - Volume 1: ICAMBBE,
TI - Therapy of Fermented Milk Lactobacillus Casei Strain Shirota to Level of Malondialdehyde (MDA) and Proteind Bands the Hearth the White Rats (Rattus Norvegicus) That given High Cholesterol Dietary
SN - 978-989-758-483-1
AU - Putri D.
AU - Aulanni’am A.
AU - Srihardyastuti A.
PY - 2019
SP - 126
EP - 132
DO - 10.5220/0009586901260132