The Simple Method for Immunohistochemistry (IHC) Analyzing of Intensity using Corel Photo-Paint® with CMYK Split-channel

Fajar Shodiq Permata, Dyah Ayu Oktavianie Ardhiana Pratama, Albiruni Haryo

2019

Abstract

Immunohistochemistry (IHC) is a popular method to detect protein in the tissue. But, determining protein level in tissue is using ELISA which needs more cost. Although, the intensity of IHC color relates to an amount in tissue. Therefore, image processing to measure the intensity of IHC quantitively was developed using much software. The common software used in Indonesia is Corel Photo-Paint® (CCP). This article described the simple method to measure the IHC intensity using CCP based on Cyan-Magenta-Yellow- Black (CMYK) Split channel. The conclusions were CCP could be used for image processing software to measure the IHC intensity. The higher intensity has fewer grey level, the lower intensity has a higher grey level.

Paper Citation

in Harvard Style

Permata F., Pratama D. and Haryo A. (2019). The Simple Method for Immunohistochemistry (IHC) Analyzing of Intensity using Corel Photo-Paint® with CMYK Split-channel.In Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering - Volume 1: ICAMBBE, ISBN 978-989-758-483-1, pages 53-57. DOI: 10.5220/0009587000530057

in Bibtex Style

@conference{icambbe19,
author={Fajar Shodiq Permata and Dyah Ayu Oktavianie Ardhiana Pratama and Albiruni Haryo},
title={The Simple Method for Immunohistochemistry (IHC) Analyzing of Intensity using Corel Photo-Paint® with CMYK Split-channel},
booktitle={Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering - Volume 1: ICAMBBE,},
year={2019},
pages={53-57},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0009587000530057},
isbn={978-989-758-483-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering - Volume 1: ICAMBBE,
TI - The Simple Method for Immunohistochemistry (IHC) Analyzing of Intensity using Corel Photo-Paint® with CMYK Split-channel
SN - 978-989-758-483-1
AU - Permata F.
AU - Pratama D.
AU - Haryo A.
PY - 2019
SP - 53
EP - 57
DO - 10.5220/0009587000530057