Antibiotic Sensitivity Test Gentamicin in Bacteria Staphylococcus aureus with Incubation Temperature 33ºC and 35ºC

Vincentia Ade Rizky, Mei Rositasari, Visensius Krisdianilo, Julia Damayanti, Sa'adah Siregar

2019

Abstract

Staphylococcus aureus is one of the bacteria that causes infection but is very resistant to various antibiotics Many factors affect the results of this antibiotic sensitivity test, one of which is the temperature factor. According to CLSI (2014) the incubation temperature for the sensitivity test used was 35 ± 2ºC, whereas according to WHO (2004) the temperature used for this test was 35ºC temperature, temperatures higher than 35ºC of the culture seemed sensitive, whereas at temperatures lower than 35ºC resistant colonies will grow inside the inhibition zone. This type of research is analytic observation. The research sample used was a pure strain of Staphylococcus aureus ATCC 25923. The study sample was taken after fulfilling the inclusion and exclusion criteria. Staphylococcus aureus bacteria that have been inoculated were planted on the Mueller Hinton Agar media and antibiotics were added by the Kirby-Bauer method, then incubated at 33ºC and 35ºC respectively. The inhibition zone yield is measured in mm. The measurement data then tested the hypothesis by the Wilcoxon test with a 0.05 consecutive confidence level. The results showed the mean zone of inhibition at 33ºC gentamicin 21.67 mm while at 35ºC gentamicin 21.67 mm. These data were analyzed and showed no difference in the zone of inhibition of Staphylococcus aureus at 33°C and 35°C incubation temperatures. Based on the results of the study it can be concluded that the difference in incubation temperature of 33ºC and 35ºC can be done in the examination of the Susceptibility Test because there is no difference between the results of the zone of inhibition of Staphylococcus aureus bacteria with variations in incubation temperature.

Paper Citation

in Harvard Style

Ade Rizky V., Rositasari M., Krisdianilo V., Damayanti J. and Siregar S. (2019). Antibiotic Sensitivity Test Gentamicin in Bacteria Staphylococcus aureus with Incubation Temperature 33ºC and 35ºC.In Proceedings of the International Conference on Health Informatics and Medical Application Technology - Volume 1: ICHIMAT, ISBN 978-989-758-460-2, pages 528-535. DOI: 10.5220/0009974305280535

in Bibtex Style

@conference{ichimat19,
author={Vincentia Ade Rizky and Mei Rositasari and Visensius Krisdianilo and Julia Damayanti and Sa'adah Siregar},
title={Antibiotic Sensitivity Test Gentamicin in Bacteria Staphylococcus aureus with Incubation Temperature 33ºC and 35ºC},
booktitle={Proceedings of the International Conference on Health Informatics and Medical Application Technology - Volume 1: ICHIMAT,},
year={2019},
pages={528-535},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0009974305280535},
isbn={978-989-758-460-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the International Conference on Health Informatics and Medical Application Technology - Volume 1: ICHIMAT,
TI - Antibiotic Sensitivity Test Gentamicin in Bacteria Staphylococcus aureus with Incubation Temperature 33ºC and 35ºC
SN - 978-989-758-460-2
AU - Ade Rizky V.
AU - Rositasari M.
AU - Krisdianilo V.
AU - Damayanti J.
AU - Siregar S.
PY - 2019
SP - 528
EP - 535
DO - 10.5220/0009974305280535