Extraction of Pectin from Durian Rind and Its Minimum Inhibitory Concentration towards Staphylococcus Aureus and Escherichia Coli

Mufti Ghaffar, Harsi Kusumaningrum, Nugraha Suyatma

2019

Abstract

Durian is a big tropical fruit grown in Indonesia that leaves many waste from its rind. Recently, some reports shows that durian rind can be extracted for its pectin that showing a potential antimicrobial activity toward pathogen. The aim of this study was to characterized durian pectin isolate from two different extraction methods and to determine the minimum inhibitory concentration (MIC) of durian pectin isolate against pathogenic bacteria. Inhibitory activity of durian pectin isolate against two bacterial strain: Staphylococcus aureus and Escherichia coli, was determined by using macrodilution methods, and amoxicilline used as positive control. Results showed that water content of the two extraction methods resulted in around 96%. Yield of durian pectin isolate in methods A (5,45 ± 0,10 %) more than extraction B (3,46 ± 0,06 %). MIC of isolates durian pectin in mueller hinton broth (MHB) medium against E. coli was 500 mg/ml and S. aureus was 110 mg/ml, this concentration showed no sign of bacterial growth, this condition was same as positive control.

Paper Citation

in Harvard Style

Ghaffar M., Kusumaningrum H. and Suyatma N. (2019). Extraction of Pectin from Durian Rind and Its Minimum Inhibitory Concentration towards Staphylococcus Aureus and Escherichia Coli. In Proceedings of the 2nd SEAFAST International Seminar - Volume 1: 2nd SIS, ISBN 978-989-758-466-4, pages 72-76. DOI: 10.5220/0009978600002833

in Bibtex Style

@conference{2nd sis19,
author={Mufti Ghaffar and Harsi Kusumaningrum and Nugraha Suyatma},
title={Extraction of Pectin from Durian Rind and Its Minimum Inhibitory Concentration towards Staphylococcus Aureus and Escherichia Coli},
booktitle={Proceedings of the 2nd SEAFAST International Seminar - Volume 1: 2nd SIS,},
year={2019},
pages={72-76},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0009978600002833},
isbn={978-989-758-466-4},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 2nd SEAFAST International Seminar - Volume 1: 2nd SIS,
TI - Extraction of Pectin from Durian Rind and Its Minimum Inhibitory Concentration towards Staphylococcus Aureus and Escherichia Coli
SN - 978-989-758-466-4
AU - Ghaffar M.
AU - Kusumaningrum H.
AU - Suyatma N.
PY - 2019
SP - 72
EP - 76
DO - 10.5220/0009978600002833