Low-dose of Acetylsalicylic Acid Upregulates Expression of eNOS mRNA and Downregulates Interleukin-6 (IL-6) and Transforming Growth Gactor-β1 (TGF-β1) mRNA in Rat Kidney of Preeclampsia Model

Yuyun Nailufar, Rul Afiyah Syarif, Andi Fitriani Kusuma, Farmita Chairani, Nia Marlina, Charolina Vivi Vienetta, M. Hadri Ar-Ridho, Totok Utoro, Nur Arfian

2020

Abstract

Preeclampsia leads to endothelial dysfunction and kidney injury with inflammation and fibrosis. Low-dose acetylsalicylic acid (ASA) administration may decrease uterine artery resistance; however, its effect on the kidney has not been elucidated yet. We performed a preeclampsia model in pregnant female Wistar rats (PE group, n=5, 150-200 grams) using L-NAME 50mg/kg of BW intraperitoneal injection on day 1-18 of pregnancy. Low doses of ASA with dose 75 (PE+ASA75) and 125 (PE+ASA125) mg/kg body weight were administered in preeclampsia rats in the day 10-12 day of pregnancy. The Control group was normal pregnant rats with NaCl treatment (n=5). On day 18, rats were sacrificed; kidneys were harvested, then extracted for Reverse Transcriptase-PCR (RT-PCR) of eNOS, TGF-β1, and IL-6 mRNA expression measurements. Proteinuria and rat blood pressure were measured before termination. L-NAME injection-induced preeclampsia showed significantly higher systolic blood pressure and proteinuria in the PE group than in the control group (p<0.05). However, there were no changes in podocin and nephrin expression. In conclusion, the low dose of ASA 125mg/Kg BW ameliorates kidney inflammation and TGF-β1 expression in the rat preeclampsia model's kidney.

Paper Citation

in Harvard Style

Nailufar Y., Syarif R., Kusuma A., Chairani F., Marlina N., Vienetta C., Ar-Ridho M., Utoro T. and Arfian N. (2020). Low-dose of Acetylsalicylic Acid Upregulates Expression of eNOS mRNA and Downregulates Interleukin-6 (IL-6) and Transforming Growth Gactor-β1 (TGF-β1) mRNA in Rat Kidney of Preeclampsia Model.In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 63-68. DOI: 10.5220/0010487800630068

in Bibtex Style

@conference{jimc20,
author={Yuyun Nailufar and Rul Afiyah Syarif and Andi Fitriani Kusuma and Farmita Chairani and Nia Marlina and Charolina Vivi Vienetta and M. Hadri Ar-Ridho and Totok Utoro and Nur Arfian},
title={Low-dose of Acetylsalicylic Acid Upregulates Expression of eNOS mRNA and Downregulates Interleukin-6 (IL-6) and Transforming Growth Gactor-β1 (TGF-β1) mRNA in Rat Kidney of Preeclampsia Model},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={63-68},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010487800630068},
isbn={978-989-758-499-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - Low-dose of Acetylsalicylic Acid Upregulates Expression of eNOS mRNA and Downregulates Interleukin-6 (IL-6) and Transforming Growth Gactor-β1 (TGF-β1) mRNA in Rat Kidney of Preeclampsia Model
SN - 978-989-758-499-2
AU - Nailufar Y.
AU - Syarif R.
AU - Kusuma A.
AU - Chairani F.
AU - Marlina N.
AU - Vienetta C.
AU - Ar-Ridho M.
AU - Utoro T.
AU - Arfian N.
PY - 2020
SP - 63
EP - 68
DO - 10.5220/0010487800630068