Antibiofilm Activity of Aloe barbadensis Miller Extract Against Staphylococcus aureus

Kanesti Ismirajna, Irena Agustiningtyas

2020

Abstract

Background: Aloe barbadensis Miller is a type of plant that can potentially inhibit the growth of bacteria causing infections. Improper use of antibiotics can result in the occurrence of bacterial resistance caused by the formation of biofilms on a living creature. The goal of this study was to investigate the antibiofilm activity of Aloe barbadensis Miller against the biofilm produced by Staphylococcus aureus. This study is classified as an experimental in vitro with the post-test only control group design. The Aloe barbadensis Miller rind macerated with methanol solvent. The extract was divided into concentrations of 20%, 10%, 5%, 2.5%, 1.25% and 0.63%, tested against biofilm formation by Staphylococcus aureus ATCC 25923. The antibiofilm activity was tested by microtiter plate biofilm assay method with 3 replications on a 96 well round-bottom microplate. Then observed by calculating the value of optical density using a microplate reader (λ = 620 nm). Extract concentrations 20%, 10%, 5%, 2.5%, 1.25% and 0.63% showed a minimum percentage of inhibition of biofilm concentration were -30.3%; 10.9%; 10.5%; 45.9%; 19.3% and 16.3% respectively. Statistical analysis using One-Way ANOVA (p = 0.008) followed by post-hoc LSD which showed a concentration of 2.5% had no significant difference (p > 0.05) with positive control using ciprofloxacin. Although the methanol extract of Aloe barbadensis Miller rind showed inhibitory activity against S. aureus biofilms was equivalent to positive control but had a Minimum Biofilm Inhibition Concentration (MBIC50) percentage <50%.

Paper Citation

in Harvard Style

Ismirajna K. and Agustiningtyas I. (2020). Antibiofilm Activity of Aloe barbadensis Miller Extract Against Staphylococcus aureus.In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 84-90. DOI: 10.5220/0010488200840090

in Bibtex Style

@conference{jimc20,
author={Kanesti Ismirajna and Irena Agustiningtyas},
title={Antibiofilm Activity of Aloe barbadensis Miller Extract Against Staphylococcus aureus},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={84-90},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010488200840090},
isbn={978-989-758-499-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - Antibiofilm Activity of Aloe barbadensis Miller Extract Against Staphylococcus aureus
SN - 978-989-758-499-2
AU - Ismirajna K.
AU - Agustiningtyas I.
PY - 2020
SP - 84
EP - 90
DO - 10.5220/0010488200840090